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ATAD2 is a Potential Immunotherapy Target for Patients with Small Cell Lung Cancer Harboring HLA-A*0201

ABSTRACT: To identify the immune peptides in SCLC tumour cells that can be processed and presented by HLA-I, we performed LC–MS/MS analysis on 11 SCLC cell lines, representing the heterogeneity of SCLC subtypes. Ten of the cell lines were confirmed positive for HLA-I, and one was HLA-I-deficient. To analyse cell surface antigens on the HLA-I-deficient cells, PBMCs isolated from healthy blood donors were treated with DMS53 cell lysate and matured into DCs to present the tumour cell antigens.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jie Wang

PROVIDER: PXD058303 | iProX | Tue Nov 26 00:00:00 GMT 2024

REPOSITORIES: iProX

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ATAD2 is a potential immunotherapy target for patients with small cell lung cancer harboring HLA-A∗0201.

Yuan Li L Li Sini S Zhu Yixiang Y Yang Lin L Zhang Xue X Qu Yan Y Wang Zhijie Z Duan Jianchun J Zhong Jia J Tian Yanhua Y Liu Lihui L Sun Boyang B Fei Kailun K Liu Zheng Z Zhang Jian J He Yan Y Guo Yufeng Y He DanMing D Zhuang Wei W Zhang Jinsong J Ma Zixiao Z Bai Hua H Wang Jie J

EBioMedicine 20250113

<h4>Background</h4>Small cell lung cancer (SCLC) represents a highly aggressive neuroendocrine tumour with a dismal prognosis. Currently, the identification of a specific tumour antigen that can facilitate immune-based therapies for SCLC remains elusive.<h4>Methods</h4>We employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) to analyse cancer/testis antigens (CTAs) in SCLC cell lines and human tumour specimens. Immunohistochemistry of clinical specimens was performed to compare prot ...[more]

PMID: 39808946

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