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Integrated proteomics and metabolomic analyses reveal the accumulation mechanism of bioactive components in Polygonatum odoratum

ABSTRACT: Polygonatum odoratum (MILL.) DRUCE is rich in bioactive components with high medicinal value. To maximize the clinical benefits, it is of great significance to efficiently extract key bioactive components from appropriate growth stages in which they are most abundant. In this study, we analyzed the changes of metabolite accumulation and protein expression in P. odoratum rhizomes at different growth stages using targeted metabolomics combined with proteomics, and identified a total of 1,237 differentially abundant metabolites (DAMs). Flavonoids accumulated most in winter, and the biosynthesis pathways associated with flavonoids, isoflavonoids, flavones and flavonols exhibited significant differentially expressed proteins (DEPs). Among them, PGT, FLS, CYP75B1, HIDH, IF7MAT, and UFT73C6 were positively correlated with flavonoid accumulation. Steroid saponins accumulated most in spring, and the biosynthetic pathways of steroid and brassinosteroid biosynthesis exhibited DEPs. Among them, FDFT1, TM7SF2, DHCR7, CAS1

ORGANISM(S): Polygonatum Odoratum

SUBMITTER: Shuang Liang

PROVIDER: PXD058887 | iProX | Sat Dec 14 00:00:00 GMT 2024

REPOSITORIES: iProX

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Integrated metabolomics and proteomics analysis reveals the accumulation mechanism of bioactive components in Polygonatum odoratum.

Liang Shuang S Zhao Yang Y Luo Zhaohui Z Liu Junchen J Jiang Xicen X Yang Bingxian B Zhang Lin L Fu Hongwei H Zhong Zhuoheng Z

Frontiers in plant science 20241220

Polygonatum odoratum (Mill.) Druce is rich in bioactive components with high medicinal value. To maximize the clinical benefits, it is of great significance to efficiently extract key bioactive components from appropriate growth stages in which they are most abundant. In this study, we analyzed the changes of metabolite accumulation and protein expression in P. odoratum rhizomes at different growth stages using targeted metabolomics combined with proteomics, and identified a total ...[more]

PMID: 39759229

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Integrated proteomics and metabolomic analyses reveal the accumulation mechanism of bioactive components in Polygonatum odoratum

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Integrated metabolomics and proteomics analysis reveals the accumulation mechanism of bioactive components in <i>Polygonatum odoratum</i>.

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