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Isolation and identification of salivary glycoproteins recognized by the lectin DSA in patients with HBV-induced cirrhosis

ABSTRACT: Early diagnosis of liver cirrhosis induced by Hepatitis B virus (HBV) using salivary biomarkers has significant implications for early detection, screening, and prognosis, with the advantages of being non-invasive, easily accessible, and cost-effective. Identifying liver cirrhosis at an early stage can greatly improve patient outcomes by enabling timely intervention, reducing the risk of progression to hepatocellular carcinoma (HCC), and enhancing the effectiveness of therapeutic strategies. Our previous study has investigated the alterations in salivary glycopatterns related to HBVinfected chronic liver diseases, and found that the tri-/tetra-antennary N-glycancontaining glycoproteins recognized by the lectin Datura Stramonium Agglutinin (DSA) was closely related to HBV-induced cirrhosis. This project used 120 salivary samples which were divided into healthy control, hepatitis B, hepatic cirrhosis, and hepatocellular carcinoma to perform saliva microarray test (individual). Furtherly, the mixed saliva of each group was isolated by the lectin DSA and then identified by LC-MS, and the N-/O-glycans were released and identified by MALDI-TOF-MS.

ORGANISM(S): Homo Sapiens

SUBMITTER: Zheng Li

PROVIDER: PXD058931 | iProX | Mon Dec 16 00:00:00 GMT 2024

REPOSITORIES: iProX

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Project description:Background: Of Hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC), 85%-90% of cases develop from liver cirrhosis, and circular RNAs (circRNAs) have important roles in this process; however, differences in serum circRNA expression profiles between patients with HBV-related cirrhosis and those with HCC have not been studied. Methods: Serum RNA was extracted from patients with HCC and cirrhosis (n = 5 per group) and used for microarray analysis of circRNA expression profiles. Bioinformatics analyses, including clustering, differential expression, and construction of a ceRNA network, were performed. Quantitative real-time reverse transcription PCR validation analysis was conducted using samples from patients with HBV-related cirrhosis (n = 88) and HCC (n = 73). Further, statistical analyses were used to analyze the potential function and value of selected circRNAs with expression differing between the HBV-related cirrhosis and HCC groups. Results: Cluster analysis revealed 8 up-regulated and 80 down-regulated circRNAs. Further, qRT-PCR analysis showed that circRNA_0000367 expression was consistent with that detected by microarray experiments, with significantly lower levels in patients with HBV-related HCC than those with HBV-related cirrhosis. CircRNA_0000367 expression levels were also significantly lower in patients with drug-resistant HBV and those with HBV-related cirrhosis with Model for End-Stage Liver Disease score < 10. Further, circRNA_00000367 expression levels were lower in patients with HBV-related cirrhosis who progressed to HCC. Analysis of the lncRNA-miRNA-mRNA ceRNA network identified 39 miRNAs and 24 mRNAs involved in circRNA_00000367 networks; these target genes were involved in various biological processes and signaling pathways. Conclusion: Serum cicrRNA_0000367 is a potential HCC biomarker in patients with HBV-related cirrhosis, where down-regulation of cicrRNA_0000367 in HBV-related cirrhosis may be associated with progression to HCC.

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Isolation and identification of salivary glycoproteins recognized by the lectin DSA in patients with HBV-induced cirrhosis

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