Dataset Information

Proteomic analysis of undescended testis

ABSTRACT: A total of 62022 unique peptides were acquired, and 7137 proteins were confidently identified and quantified inthe cryptic (CT) and contralateral (CLT) testis from four unilaterally cryptorchid rams

ORGANISM(S): Ovis Aries

SUBMITTER: Xiangpeng Yue

PROVIDER: PXD059032 | iProX | Thu Dec 19 00:00:00 GMT 2024

REPOSITORIES: iProX

ACCESS DATA

Publications

Identification of key genes related to unilateral cryptorchidism in sheep by comprehensive transcriptomics and proteomics analyses.

Pei Sheng-Wei SW Liu Yang-Kai YK Wang Zhong-Yu ZY Yuan Ze-Hu ZH Li Wan-Hong WH Li Fa-Di FD Yue Xiang-Peng XP

BMC genomics 20250219 1

<h4>Background</h4>Cryptorchidism is one of the most common reproductive abnormalities in rams, which seriously harms the reproductive capacity of rams and causes significant economic losses to the sheep industry. However, there are few studies elucidating its hereditary predisposition in sheep.<h4>Results</h4>In the present study, the transcriptome and proteome of the cryptic (CT) and contralateral (CLT) testis from four unilaterally cryptorchid rams, and the normal testis (NT) from four health ...[more]

PMID: 39972276

Similar Datasets

Project description:Background Clinical success of T cell receptor (TCR) gene therapy has previously been demonstrated for NY-ESO-1 TCR gene therapy. To increase numbers of cancer patients that can be treated with TCR gene therapy we aimed to identify a novel set of high-affinity cancer specific TCRs targeting different cancer testis (CT) antigens in prevalent HLA class I alleles. Methods In this study, we selected based on publicly available gene expression databases the most promising CT genes to target. From these selected genes we identified by HLA peptidomics the naturally processed and presented HLA class I peptides. With these peptide-HLA tetramers were generated, and by single cell sorting CT specific CD8+ T cells were selected, and expanded from the allo-HLA repertoire of healthy donors. By several functional assays high avidity CT-specific T cell clones with safe recognition pattern were selected. To evaluate the potential for clinical application in TCR gene therapy, TCRs were sequenced and transferred into peripheral blood derived CD8+ T cells. Results In total we identified, 7 novel CT-specific TCRs that effectively target MAGE-A1, MAGE-A3, MAGE-A6 and MAGE-A9 in the context of human leukocyte antigen(HLA) -A1, -A2, -A3, -B7, -B35 and -C7. TCR gene transfer into CD8⁺ T cells resulted in efficient cytokine production and cytotoxicity of variety of different tumor types without detectable cross-reactivity. In addition, major in vivo antitumor effects of MAGE-A1 specific TCR engineered CD8⁺ T cells was observed in an orthotopic xenograft model for established multiple myeloma, in which bone marrow located tumor cells were completely eradicated after T cell injection. Conclusion The identification of 7 novel CT-specific TCRs, reactive against CT antigens presented in a variety of different HLA class I alleles, allows selection of therapeutic TCRs for an increased number of cancer patients, and will improve development of personalized TCR gene therapy.

Dataset Information

Proteomic analysis of undescended testis

Publications

Identification of key genes related to unilateral cryptorchidism in sheep by comprehensive transcriptomics and proteomics analyses.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets