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In-depth Spatial Proteomics for Whole Tissue Profiling by Sparse Sampling Strategy

ABSTRACT: To achieve a deep quantitative proteomic profiling of whole tissue with the spatial localization preserved, we designed an untargeted spatial proteomics workflow with a sparse sampling strategy. We combined a computational approach and MS-based proteomics analysis to localize high-throughput proteome mapping within a tissue by microdissecting the slices into a series of parallel strips with different angles, that allow subsequent image reconstruction. We successfully reconstructed a whole-tissue mapping in a mouse brain, consisting of 8,645 protein distributions and expressions with a spatial resolution of approx. 300 μm.

ORGANISM(S): Mus Musculus

SUBMITTER: Weijie Qin

PROVIDER: PXD059321 | iProX | Tue Dec 31 00:00:00 GMT 2024

REPOSITORIES: iProX

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