Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of novel interacting proteins of ANLN

ABSTRACT: ANLN is a mitotic protein that is overexpressed in a variety of cancers. In order to reveal the function of ANLNanln in esophageal squamous cell carcinoma (ESCC) cells, this study prepared ANLN interacting protein profiles by enriching ANLN proteins in ESCC cell lines (kyse150 cells) to identify and study key ANLN interacting proteins.

ORGANISM(S): Homo Sapiens

SUBMITTER: Enmin Li

PROVIDER: PXD059453 | iProX | Mon Jan 06 00:00:00 GMT 2025

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Proteome of ESCC identified by LC-MS/MS

Project description:Protein identification in esophageal squamous cell carcinoma(ESCC) was performed,we explored the proteomic landscape in ESCC and attempted to perform clinical-related patient classification based on ESCC proteome.

2026-06-15 | PXD053809 | Pride

Gene expression in normal and tumoral esophageal squamous cell lines

Project description:This study was designed to identify genes aberrantly expressed in esophageal squamous cell carcinoma (ESCC) cells. Three esophageal squamous cell carcinoma-derived cell lines and one normal human esophageal squamous cell line were analyzed.

2014-09-20 | E-GEOD-61587 | biostudies-arrayexpress

RNAi profiling of human fascin knockdown esophageal squamous carcinoma cells

Project description:Fascin, an actin-binding protein, is upregulated in a variety of cancers, including esophageal squamous cell carcinoma (ESCC) and is proposed to function in cellular growth, mobility and invasiveness. Nonetheless, the molecular mechanisms through which fascin expression contributes to the proliferation and invasiveness of ESCC are unknown. To address this issue and to identify genes and biological pathways associated with fascin expression, a genome-wide gene expression analysis was performed on a fascin RNAi-depleted cell line and a corresponding ESCC cell line previously determined to express high levels of fascin. Experiment Overall Design: We used cDNA microarrays to identify genes that were differentially expressed upon fascin knockdown. For this purpose, we selected PSF8 (Fascin siRNA-depleted cell) and PSC(blank vector control cell ), two ESCC cell lines recently established in our laboratory. Total RNA from PSF8 cells and PSC cells was prepared for use with Affymetrix U133 cDNA microarrays.

2008-06-20 | E-GEOD-11373 | biostudies-arrayexpress

The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (Affymetrix microarray analysis)

2014-01-09 | GSE53892 | GEO

Regulatory mechanism of HNRNPA2B1 in human esophageal squamous cell carcinoma cells

Project description:HNRNPA2B1 regulates the expression and alternative splicing of apoptosis related genes with esophageal squamous cell carcinoma development in KYSE150 cells

2026-01-09 | E-MTAB-16311 | biostudies-arrayexpress

Fusobacterium nucleatum FadA promotes the progression of esophageal squamous cell carcinoma through binding to TFRC

Project description:In China, esophageal squamous cell carcinoma (ESCC) represents the major histopathological form of esophageal cancer. Fusobacterium nucleatum (F. nucleatum, Fn) has been reported to be associated with ESCC. FadA has been identified exclusively in F. nucleatum. However, the potential role and underlying mechanism of FadA in ESCC remain unclear. Whole genome sequencing (WGS) data of ESCC tissue from NCBI database were analyzed to characterize the microbial composition of ESCC. The relative expression of Fn and FadA was validated by qPCR in another ESCC tissue cohort. An E. coli BL21 (DE3) expression system was utilized to express FadA. After purification, FadA was co-cultured with KYSE150 cells. Cell migration, proliferation, and invasion were evaluated by wounding-healing, CCK-8, and Transwell migration and invasion assays. RNA and proteomic sequencing analysis were performed to identify the altered molecule initiated by FadA treatment. TFRC was further validated by knockout and overexpression in KYSE150 cells. Molecular docking, molecular dynamics simulations, and normal mode analysis (NMA) were used to predict and evaluate the interaction between FadA and TFRC. ESCC tissue exhibited decreased α-diversity and distinct microbial composition, with significant enrichment in F. nucleatum. FadA expression was significantly elevated in ESCC tissue samples. Functional assays illustrated that FadA promoted the migration, proliferation, and invasion of ESCC cells. Integrated RNA and proteomic sequencing identified TFRC as a key upregulated target after FadA treatment, which was further confirmed by TFRC overexpression and knockout. Computational analysis supported a stable interaction between FadA and TFRC. FadA is upregulated in ESCC and promotes the migration, proliferation, and invasion in ESCC through TFRC. FadA may serve as a potential biomarker and therapeutic target in ESCC.

2026-04-04 | GSE326337 | GEO

Differential protein expression between ESCC and normal tissues

Project description:To understand the difference of protein expression between paired esophageal squamous cell carcinoma (ESCC) and adjacent normal tissues, we collected 10 paired ESCC and normal tissues from surgical resected specimems for high-throughput proteomic experiments. From comparative analysis, the dysregulated signaling pathways in ESCC could be uncovered.

2020-08-11 | PXD019834 | Pride

The Demethylase JMJD2C/KDM4C Localizes to H3K4me3 Positive Transcription Start Sites (Affymetrix microarray analysis)

Project description:We have mapped transcriptional changes after depletion of the histone demethylases JMJD2C/GASC1/KDM4C and JMJD2A/KDM4A alone or in combination in the esophageal squamous carcinoma cell line, KYSE150. The KYSE150 cell line contains an amplification of the JMJD2C locus. RNA was extracted from KYSE150 cells transfected with shRNAs targeting JMJD2C and/or JMJD2A. The experiment was performed in triplicates and expression levels analyzed using Affymetrix microarrays.

2014-01-09 | E-GEOD-53892 | biostudies-arrayexpress

Microarray expression profile and functional analysis of circular RNAs in plasma for Esophageal Squamous Cell Carcinoma

Project description:The purpose of this study is to explore the circRNAs expression profiles in the plasma from esophageal squamous cell carcinoma (ESCC) patients.The purpose of this study is to explore the circRNAs expression profiles in the plasma from esophageal squamous cell carcinoma (ESCC) patients.

2021-03-23 | GSE112496 | GEO

Effect of depletion of RBM4 on gene expression of esophageal squamous cell carcinoma cell KYSE150

Project description:To explore the role of RBM4 in esophageal squamous cell carcinoma cell,we performed high-throughput mRNA sequencing (mRNA-seq) in KYSE150 with stable knockdown of RBM4 by shRNA . We then performed gene expression profiling analysis using data obtained from RNA-seq of KYSE150 cells,three replicates were sequenced.

2023-04-26 | GSE220613 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data