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Pacific oyster shellome_three distinct strategies (LC-MS/MS, PF-LC-MS/MS, and Astral mass spectrometer)

ABSTRACT: We expanded the shell mass used for the extraction and identification of shell matrix proteins to 300g, three distinct strategies (LC-MS/MS, PF-LC-MS/MS, and Astral mass spectrometer) were used to obtain the most complete shell matrix protein library of Crassostrea gigas

ORGANISM(S): Crassostrea Gigas

SUBMITTER: Li Li

PROVIDER: PXD059800 | iProX | Tue Jan 14 00:00:00 GMT 2025

REPOSITORIES: iProX

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The Atlas of the Shell Proteome in Oysters Reveals the Potential Roles of the Cytoskeleton and Extracellular Matrix in Biomineralization.

Yang Qi Q Wang Shentong S Liu Mingkun M Wang Wei W Zhang Guofan G Li Li L

Journal of proteome research 20250908 10

Shell matrix proteins (SMPs) are fundamental biological macromolecules for mollusk shell formation, yet fewer than 400 SMPs in mollusks have been previously identified, hindering our understanding of how mollusks construct and maintain their shells. Here, we identified 1689 SMPs in the Pacific oyster <i>Crassostrea gigas</i> using three different mass spectrometry techniques, representing a significant methodological advancement in shell proteomics, enabling a 6.52-fold increase in SMP identific ...[more]

PMID: 40919894

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