Heat shock chaperone HSPB1 mitigates poly-glycine-induced neurodegeneration via restoration of autophagic flux
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ABSTRACT: HEK293T cells were transfected with GFP-poly-G-TurboID or GFP-TurboID as a control. Following biotin labelling, the cells were lysed in RIPA buffer to separate the soluble and insoluble fractions. The insoluble proteins in the pellet containing poly-G aggregates were further lysed in the lysis buffer containing 8 M urea to ensure effective solubilization
ORGANISM(S): Homo Sapiens
SUBMITTER:
Lei Li
PROVIDER: PXD059955 | iProX | Mon Jan 20 00:00:00 GMT 2025
REPOSITORIES: iProX
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