Project description:After injecting various strains of the rabies virus into the brains of mice, we conducted proteomic analysis of the brain tissue under different disease conditions.
Project description:Foxp3+ regulatory T cells (Tregs) are critical components of immune tolerance. In addition, Tregs residing in non-immune tissues perform specialized functions in tissue homeostasis and remodeling. The characteristics and functions of brain Tregs, however, are not well understood, in part because the number of Tregs in the brain under normal conditions is very low. However, during the chronic phase two weeks after a stroke caused by ischemic brain injury, a massive accumulation of Tregs occurs in the brain. We found that brain Tregs differ from Tregs in other tissues such as adipose tissue (VAT) and muscle Tregs.
Project description:The 10 samples below represent a study where gene expression levels were measured in 5 different parts of the rat brain under cocaine-treated (GSM4696, GSM4698 - GSM4701) and saline-treated control (GSM4702 - GSM4706) conditions. The regions studied were amygdala (amy), caudate putamen (cpu), nucleus acumbens (na), prefrontal cortex (cpu), and the ventral tegmental area (vta). The data were analyzed using two different computational techniques, viz. singular value decomposition (SVD) and self-organizing maps (SOM), to identify a common set of genes that were regulated by cocaine administration.
Project description:DrugMatrix is a comprehensive rat toxicogenomics database and analysis tool developed to facilitate the integration of toxicogenomics into hazard assessment. Using the whole genome and a diverse set of compounds allows a comprehensive view of most pharmacological and toxicological questions and is applicable to other situations such as disease and development. Complete Drug Matrix dataset for rat brain. Approximately 600 different compounds were profiled in up to 8 different rat tissues by obtaining tissue samples from test compound-treated and vehicle control-treated rats in biological triplicates for gene expression analysis after 0.25, 1, 3, and 5 days of exposure with daily dosing. In a few studies (1.8%), 7 days of exposure was substituted for 5 days of exposure. Samples were hybridized to CodeLink RU1 10K rat arrays (Amersham Biosciences).
Project description:Tissue Top-down microproteomic was performed on 3 brain regions. 156 references proteins from different cellular compartments were characterized. Moreover, 11 Alternative proteins issued from alternative open reading frames (AltORF) were identified and were relied to the brain regions and associated to the function of their reference proteins. Some proteins display specific post-translational modifications profiles or truncation linked the brain regions and their functions. Systemic biology performed on microproteome identified in each region allowed to associate sub-networks with functional physiology of each brain region. Back correlation of the local extracted and identified microproteome with tissue cellular localization was then performed by MALDI mass spectrometry imaging. 40 proteins including 4 Altproteins have been back correlated in MALDI MSI and are in line with their tissue extracted region and physiological function. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
Project description:Maldi imaging with NEDC matrix of a rat brain tissue section. Image was acquired with 50 um resolution. Ion mobility seperation enabled. Negative ion mode.