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IP-mass spec GFP and GFP-CFAP100

ABSTRACT: HaCaT cells were transfected with CFAP100- 11 GFP, and the lysate was subjected to immunoprecipitation with the anti-GFP antibody

ORGANISM(S): Homo Sapiens

SUBMITTER: Shuang Sun

PROVIDER: PXD061110 | iProX | Mon Feb 24 00:00:00 GMT 2025

REPOSITORIES: iProX

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CFAP100 couples microtubule glutamylation to spindle pole integrity in keratinocytes to promote epidermal development.

Sun Shuang S Wang Zhaoying Z Xu Zhaoyang Z Wang Zhengfeng Z Sun Jia J Li Keke K Liu Min M Zhao Huijie H Liu Peiwei P Zhou Jun J

Nature communications 20250701 1

Despite the importance of keratinocytes in epidermal structure and function, the molecular mechanisms regulating the division of these cells remain poorly understood. Herein, we demonstrate an essential role for cilia and flagella associated protein 100 (CFAP100) in keratinocyte division. Cfap100-knockout mice display a thinner and transparent skin and an impaired epidermal barrier function. Depletion of CFAP100 in keratinocytes prolongs mitotic progression and compromises chromosome segregation ...[more]

PMID: 40593606

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