Dataset Information

Longitudinal multi-omics analysis of convalescent individuals with respiratory sequelae 6–36 months after COVID-19

ABSTRACT: This study presents a longitudinal multi-omics analysis of COVID-19 convalescents with respiratory sequelae, spanning 6–36 months post-infection. The integrating proteomic and metabolomic profiling uncovers persistent molecular alterations, impaired diffusing capacity for carbon monoxide, and proposes a biomarker panel for predicting lung dysfunction. These findings offer insights into long-term post-COVID sequelae mechanisms.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jieqiong Li

PROVIDER: PXD061113 | iProX | Sun Feb 23 00:00:00 GMT 2025

REPOSITORIES: iProX

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Longitudinal multi-omics analysis of convalescent individuals with respiratory sequelae 6-36 months after COVID-19.

Yang Huqin H Guan Lujia L Xue Yi Y Li Xuyan X Gao Leyi L Zhang Zhijin Z Zhang Haifan H Ma Haomiao H Liu Fengjiao F Huang Xuan X Tong Zhaohui Z Li Jieqiong J

BMC medicine 20250305 1

<h4>Background</h4>Approximately 10-30% of individuals continue to experience symptoms classified as post-acute sequelae of coronavirus disease 2019 (COVID-19 (PASC)). PASC is a multisystem condition primarily characterized by respiratory symptoms, such as reduced diffusing capacity for carbon monoxide (DLco). Although many studies have investigated the pathogenesis of acute COVID-19, the long-term molecular changes in COVID-19 convalescents with PASC remain poorly understood.<h4>Methods</h4>We ...[more]

PMID: 40038650

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Project description:SARS-CoV2 infection results in clinically heterogeneous manifestations that are partially driven by complex longitudinal interactions with the immune system. The public health burden of mild COVID-19 is massive given the >100 million infected worldwide, but most research has focused on severe and fatal COVID-19. We recruited a mild COVID-19 cohort for multimodal immunophenotyping of single immune cells (scRNAseq, scATACseq, flow cytometry), serum proteins, virus-specific cellular and humoral immune responses, and clinical annotation from early acute infection to convalescence. Samples were acquired longitudinally from early acute infection before 15 days to >100 days post-symptom onset. Comparison to uninfected controls revealed marked immune activation consistent with acute response to viral infection at 15 days post-symptom onset with stronger inflammatory responses in older (>=40) compared to younger (<40) participants. Type I, II, and III interferon responses were most consistently upregulated in nearly all PBMC subsets. Plasmablasts were expanded in early infection with signatures of active IFNL and IFNG signaling, linking early inflammation and IFN responses to control of viral replication via antibodies. Longitudinal models of scRNAseq confirmed most inflammatory pathways decreased over time, including type I and II IFN signaling, as well as signaling by innate danger sensors (TLR, RIG-I) that detect viral replication. By contrast, genes with increasing expression over time were enriched for EMT and wound healing pathways. Most participants showed consistent decay of inflammatory signatures by convalescence at >d 30 post-symptom onset except those presenting with PASC. Despite similar virus-specific adaptive immune responses between post-acute sequelae PASC and recovered convalescent participants, PASC participants showed persistent serum cytokine and chemokine differences including elevated IL5, TNF, IL-12p70, and soluble CD28 at >30 d post-symptom onset. scRNAseq, scATACseq, and network analyses provided more signs of persistent inflammatory activation in multiple cell types, with multimodal evidence pointing to increased activity of innate cells (CD14 monocytes, DCs) and effector T cells (CD4 TEM, CD8 TEMRA). Cytokines at day 7 PSO were highly correlated with and predictive of both virus-specific IgG and neutralizing antibody titers. Integrative network analyses defined the most enriched ligand-receptor pathways across PBMC subsets including LTA/TNF , TNF, and IFNg in early infection, and these also showed signs of persistent activation in PASC. Common downstream targets of these pathways in PASC point to novel therapeutic targets and mechanistic hypotheses, including IL-1Ꞵ, AP-1, ZFP36/TTP, and CEBP/ꞵ. These findings revealed dynamics of immune activation and critical early signals correlated with adaptive immune responses. Serum protein signatures predicting antibody responses and correlating with PASC may provide opportunities for enhanced immunomonitoring and personalized treatment.

Dataset Information

Longitudinal multi-omics analysis of convalescent individuals with respiratory sequelae 6–36 months after COVID-19

Publications

Longitudinal multi-omics analysis of convalescent individuals with respiratory sequelae 6-36 months after COVID-19.

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