Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation

ABSTRACT: MDA-MB-231 cells with expression of Flag-GPX4 were treated with IFNg. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.

ORGANISM(S): Homo Sapiens

SUBMITTER: Daqian Xu

PROVIDER: PXD061373 | iProX | Sat Mar 01 00:00:00 GMT 2025

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation.

Zheng Peixiang P Hu Zhiqiang Z Shen Yuli Y Gu Lina L Ouyang Yuan Y Duan Yuran Y Ji Guimei G Dong Bofei B Lin Yanni Y Wen Ting T Tian Qi Q Hou Yueru Y Zhou Qimin Q Sun Xue X Chen Xiaohan X Wang Katherine L KL Luo Shudi S Wu Shiqi S Sun Yuening Y Li Min M Xiao Liwei L Wu Qingang Q Meng Ying Y Liu Guijun G Wang Zheng Z Bai Xueli X Duan Shengzhong S Ding Yuan Y Bi Yanli Y Wang Yuhao Y Li Gaopeng G Liu Xiaoguang X Lu Zhimin Z Wu Xiaohong X Tang Zhiyuan Z Xu Daqian D

Nature chemical biology 20250425 9

Tumor cells adapt to the inflammatory tumor microenvironment (TME) and develop resistance to immunotherapy, with ferroptosis being a major form of tumor cell death. However, the mechanisms by which tumor cells coordinate TME stimuli and their unique metabolic traits to evade ferroptosis and develop resistance to immunotherapy remain unclear. Here we showed that interferon-γ (IFNγ)-activated calcium/calmodulin-dependent protein kinase II phosphorylates phosphoserine aminotransferase 1 (PSAT1) at ...[more]

PMID: 40281343

Similar Datasets

Tang2019 - Pharmacology modelling of AURKB and ZAK interaction in TNBC

Project description:Aurora Kinase B and ZAK interaction model Equivalent of the stochastic model used in "Network pharmacology model predicts combined Aurora B and ZAK inhibition in MDA-MB-231 breast cancer cells" by Tang et. al. 2018. The only difference is cell division and partitioning of the components, which are available in the original model for SGNS2.

2024-09-02 | BIOMD0000000940 | BioModels

Gene expression profile in breast cancer cells

Project description:We report the gene expression patterns in MDA-MB-231 (a line selected for low metastatic ability), MDA-MB-231-1833 (its bone-tropic metastatic derivative line), MDA-MB-231p27CK-DD (a phosphomimetic cell line), MDA-MB-231-1833shp27 (p27 knockdown cell line), MDA-MB-231-1833PF1502 (PI3K inhibitor treatment). It shows that the gene expression pattern are regulated in a p27 phosphorylation-dependent manner.

2019-03-19 | GSE112445 | GEO

Exploring the mechanism of transcription factor TAF7 promoting the progression of triple-negative breast cancer

Project description:To further study the regulation of TAF7 on cells, the transcriptome sequencing on MDA MB-231 cells, MDA MB-231/shTAF7 cells, MDA 231-LM2 and MDA 231-LM2/shTAF7 cells were performed.

2025-01-20 | GSE276005 | GEO

Knockout of GPx4 gene in mouse keratinocyte

Project description:Comparative analysis of gene expression in cultured primary keratinocytes isolated from newborn control (K14-cre; GPx4fl/+) and knockout (K14-cre; GPx4fl/fl) mice. Selenoproteins are essential for skin function, as targeted abolition of selenoproteins in epidermal tissue results in newborn mice manifesting gross abnormalities of skin and hair, accompanied by retarded growth and premature death. To investigate whether lack of a single selenoprotein could induce similar phenotypic effect in mice, we generated keratinocyte-specific knockout mice lacking glutathione peroxidase 4 (GPx4), an essential selenoprotein in skin, to examine phenotypic changes resulting from the lack of GPx4 in skin. Ablation of GPx4 results in focal alopecia and disturbed hair follicle morphogenesis, with GPx4 being essential during early stages of hair follicle morphogenesis as well as for keratinocyte adhesion and proliferation in culture. We have generated mice with selective removal of the GPx4 gene in keratinocytes under the control of Keratin-14-cre (K14-cre) promoter. Comparative microarray analysis was performed on RNA samples taken from pooled primary keratinocytes from knockout and control mice from the same litter. Array replicates were performed using RNA samples from three different litters.

2012-12-06 | E-GEOD-34215 | biostudies-arrayexpress

LncRNA Uc003xsl.1 Activates of NF-κB/IL8 Axis to Promotes Triple-negative Breast Cancer Progression via A Decoy Role to NKRF [lnc231]

Project description:To discover the potential drivers of TNBC metastasis, we established an in vivo model by injecting MDA-MB-231cells into the tail veins of mice. Then, the breast tumor cells that successfully grew into metastatic lung tumors were collected and expanded in vitro, followed by re-injected into the tail veins of mice for lung metastasis. After three rounds of selection, a highly metastatic subline, MDA-MB-231-P3, was established, and more frequent micro-metastasis was detected in MDA-MB-231-P3 groups than that of MDA-MB-231 groups when the lungs of mice were stained with hematoxylin and eosin (HE). The lncRNA profiles of MDA-MB-231 or MDA-MB-231-P3 cells were analyzed by lncRNA sequencing. A total of 267 lncRNAs in MDA-MB-231-P3 cells were upregulated more than 2-fold in comparison to the MDA-MB-231 cells.

2021-11-29 | GSE163900 | GEO

GPX4 immunoprecipitation in Huh7 cells

Project description:Huh7 cells with expression of Flag-GPX4 were treated with IGF1. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.

2023-02-22 | MSV000091343 | MassIVE

Cancer-cell-secreted miR-9-5p regulates cholesterol homeostasis to promote tumor metastasis [TF TB Rab27AKD miR-9KO]

Project description:To investigate the effects of breast cancer derived EVs on liver metabolism,we inoculated MDA-MB-231,231 /Rab27A KD and 231 /miR-9 KO cells into subcutaneous tumor in NSG mice. We then performed gene expression profiling analysis using data obtained from RNA-seq of liver from mice xenografted MDA-MB-231 cells (tumor bearing) or MDA-MB-231/Rab27A KD cells (231/Rab27A KD) or MDA-MB-231 /miR-9 KO (231/miR-9 KO) and tumor free mice.

2024-08-31 | GSE222707 | GEO

Expression profiles of MDA-MB-231, MDA-231 S1a and S1b

Project description:Identification of genes that are involved in self-seeding by comparing gene expression profiles between parental MDA-MB-231 cells and seeder cells (MDA-231-S1a and S1b) 2 replicates from each sample (parental MDA-MB-231, MDA-MB-231 S1a and MDA-MB-231 S1b) were analyzed

2010-04-07 | E-GEOD-18833 | biostudies-arrayexpress

MDA-MB-231 Breast Cancer Bone Tropic vs Parental Hypoxic Secretome Analysis

Project description:The project profiled the expression patterns in hypoxia induced secretomes between MDA-MB-231 parental and MDA-MB-231 Bone Tropic (BT) breast cancer cell lines which have been previously generated by Massague and colleagues (Kang et al. Cancer Cell 2003).

2017-03-29 | MSV000080797 | MassIVE

Reduced JAG1 expression through miR-200 overexpression or Crispr-Cas mediated knockout impairs TNBC growth and metastasis.

Project description:Studies from our lab and others demonstrated that increasing miR-200 expression in TNBC cell lines reduced proliferation and invasion in vitro and tumor growth and metastasis in vivo. However, the mechanisms through which miR-200s suppress TNBC growth and metastasis remains incompletely characterized. In this study, we found that overexpression of miR-200s in MDA-MB-231 and MDA-MB-436 cells significantly reduced the expression of several Notched-Jagged genes including JAG1. To further investigate the importance of JAG1, JAG1 was knocked out using Crispr-Cas in MDA-MB-231 cells which express high endogenous JAG1. Loss of JAG1 in MDA-MB-231 cells significantly reduced proliferation and invasion as well as increasing apoptosis in vitro. Moreover, loss of JAG1 delayed mammary tumor onset, mammary tumor growth rate and metastatic dissemination in vivo. RNA sequencing of control and MDA-231JAG1KO tumors revealed that knocking out JAG1 altered the expression of genes associated with the extracellular matrix, angiogenesis, and epithelial to mesenchymal transition. These results imply that miR-200s may mediate some of their anti-tumor actions through reducing JAG1 expression. In addition, the loss of JAG1 dramatically suppressed TNBC growth and metastasis thus suggesting that agents targeting JAG1 should be further evaluated as a therapeutic strategy for TNBC.

2025-05-05 | GSE288207 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data