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Validation of α-satellite-associated regulators using the dCas9-TurboID system in HeLa cells

ABSTRACT: To validate whether the dCas9-TurboID system is applicable for proximity labeling at specific genomic regions, label-free mass spectrometry was employed to validate the satellite relative proteins that were enriched in the group of stable Hela cell line expressing sgAS.

ORGANISM(S): Homo Sapiens

SUBMITTER: Pengxu Qian

PROVIDER: PXD061518 | iProX | Wed Mar 05 00:00:00 GMT 2025

REPOSITORIES: iProX

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Selective identification of epigenetic regulators at methylated genomic sites by SelectID.

Qian Wenchang W Jiang Penglei P Niu Mingming M Fu Yujuan Y Huang Deyu D Zhang Dong D Liang Ying Y Wang Qiwei Q Han Yingli Y Zeng Xin X Shi Yixin Y Jiang Lingli L Yu Zebin Z Li Jinxin J Lu Huan H Wang Hong H Chen Baohui B Qian Pengxu P

Nature communications 20250418 1

DNA methylation is a significant component in proximal chromatin regulation and plays crucial roles in regulating gene expression and maintaining the repressive state of retrotransposon elements. However, accurate profiling of the proteomics which simultaneously identifies specific DNA sequences and their associated epigenetic modifications remains a challenge. Here, we report a strategy termed SelectID (selective profiling of epigenetic control at genome targets identified by dCas9), which intr ...[more]

PMID: 40251151

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