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CENPU promotes tumorigenesis and stem cell properties in triple-negative breast cancer by suppressing lysosomal furin degradation

ABSTRACT: Centromere protein U (CENPU), which is a critical component of the centromere complex, is involved in the cell filament. However, limited research has been conducted on the relationship between CENPU expression and tumors. Immunohistochemical assessment of clinical samples and analysis of TCGA data revealed a positive correlation between CENPU expression and the degree of invasiveness in breast cancer cells. CENPU promoted stem-cell-like behavior and tumorigenicity and induced malignancy in breast cancer cells. Mechanistically, CENPU promoted furin activity by inhibiting its lysosomal degradation. Furin, which is a precursor-processing enzyme of nerve growth factor (NGF), promoted the conversion of proNGF to NGF which could promote breast cancer stem cells in triple-negative breast cancers. . A tumorigenesis assay conducted in a mouse model showed that CENPU promoted tumorigenesis, and treatment with a furin inhibitor suppressed this effect. Our findings revealed that CENPU plays a critical role in furin-mediated signaling responsible for tumorigenesis. Therefore, CENPU may be a novel molecular target in triple-negative breast cancer.

ORGANISM(S): Homo Sapiens

SUBMITTER: Dongdong Zhou

PROVIDER: PXD061628 | iProX | Sat Mar 08 00:00:00 GMT 2025

REPOSITORIES: iProX

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Project description:Background Breast cancer is the leading cause of cancer related deaths in women. Triple negative breast cancers are highly aggressive, have an overall poor prognosis and has limited therapeutic options. Previously, we have identified an environmental induced gene mdig that predicted the overall survival in breast cancer patients and regulated breast cancer cell growth, motility and invasion partially through DNA and histone methylation. Mdig’s role in breast cancer growth and motility has been revealed however a comprehensive analysis of the proteomic profile of mdig affected cells in triple negative breast cancer has not been attempted yet. Methods We applied mass spectrometry technology featuring global proteomics and post translational modifications in triple negative breast cancer cells MDA-MB-231 that were deleted for mdig via CRISPR Cas 9 gene editing. Using label-free bottom up quantitative proteomics, we compared the proteomes of wildtype control (WT) and mdig knockout (KO) MDA-MB-231 cells and identified the proteins and pathways that are significantly altered with mdig deletion. Ingenuity Pathway Analysis (IPA) platform was further used to explore the signaling pathway networks with differentially expressed proteins and identified top canonical pathways, upstream regulators, and causal disease networks. Results 904 differentially expressed (p < 0.005) proteins were identified in MDA-MB-231 cells knocked out for mdig. Post-translational modification (PTM) analysis indicated that mdig loss reduced the abundance of oxidized histidine 39 of 60S ribosomal protein L27a (q = 0.00030) whereas global abundance of di-methylated lysine containing peptides was increased (p = 0.02). Relevance Our data provides the first insight towards the protein expression pattern in breast cancer associated with a complete disruption of the mdig gene. Differentially expressed proteins between WT and KO MDA-MB-231 triple negative breast cancer cells provided substantial information regarding the key proteins, biological process and pathways that are specifically upregulated in breast cancer tumorigenicity and invasiveness. Mdig affected signaling pathways and hub molecules will benefit for the development of novel treatment strategies for the development of breast cancer therapies.

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CENPU promotes tumorigenesis and stem cell properties in triple-negative breast cancer by suppressing lysosomal furin degradation

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