Human VSMC infected with Ad-CEMIP and immunoprecipitated with anti-CEMIP for proteomic analysis

ABSTRACT: HASMCs were infected with Ad-CEMIP. Whole cell lysates were obtained from 5×108 cells. CEMIP or IgG antibodies are added to capture CEMIP complexes in cell lysates. Agarose beads were washed with IP buffer, CEMIP antibody-binding complexes were captured by adding agarose beads to cell lysates, and non-specific binding was washed with IP buffer. The agarose beads were eluted with 1xSDS for mass Spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yi Zhu  

PROVIDER: PXD062060 | iProX | Thu Mar 20 00:00:00 GMT 2025

REPOSITORIES: iProX