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Alterations in IP6K2 Protein Interactions in HCT116 Cells Pre- and Post-Dextran Sulfate Sodium Exposure

ABSTRACT: Using HCT116 wild-type cells as a blank control, we divided 3×Flag-IP6K2 knockin HCT116 cells into two groups: a negative control (untreated) and an experimental group (treated with 3% DSS for 4 hours). After treatment, cells were lysed, and Flag-tagged IP6K2 and its interacting proteins were immunoprecipitated (IP) using anti-Flag beads. Subsequently, bound proteins were competitively eluted with a FLAG peptide. The eluates were then analyzed by mass spectrometry (MS) to identify DSS-induced alterations in the IP6K2 interactome.

ORGANISM(S): Homo Sapiens

SUBMITTER: Feng Rao

PROVIDER: PXD062286 | iProX | Wed Mar 26 00:00:00 GMT 2025

REPOSITORIES: iProX

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