Dataset Information

Candidate Arabidopsis MYB5-interacting proteins in developing siliques identified by IP-MS

ABSTRACT: 2 g developing siliques were harvested from the pMYB5:MYB5-GFP/myb5-2 complementary plants treated with 100 μM GA3 or 50 μM PAC. The 35S:GFP transgenic plants served as a negative control. Siliques were immediately crosslinked with 1% paraformaldehyde for 30 min on ice in a vacuum chamber. Total proteins were extracted using the Plant Cell lysis buffer for Western and IP (Beyotime, P0043) supplemented with phenylmethylsulfonyl fluoride (Solabio) and a proteinase inhibitor cocktail (Calbiochem). To reduce non-specific binding, protein extracts were pre-cleared with 50 μL of Binding control Agarose beads (ChromTek) for 1 h at 4 °C with gentle rotation. After brief centrifugation, the supernatant was incubated with 50 μL of GFP-Trap Agarose beads (ChromTek) for 2 h at 4 °C with gentle rotation. After 10 washes of the beads with phosphate-buffered saline, proteins on the beads were eluted through boiling in SDS-PAGE sample buffer for 10 min and then briefly separated in 10% SDS-PAGE gel. The gel regions containing proteins were cut and then subjected to LC-MS/MS analysis at Applied Protein Technology (Zhejiang). Three biological replicates were performed. The MS/MS database search was performed using the Maxquant software against the NCBI database with the default parameters (FDR < 0.01).

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Yan Xu

PROVIDER: PXD062288 | iProX | Thu Mar 27 00:00:00 GMT 2025

REPOSITORIES: iProX

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Publications

Gibberellin signaling regulates pectin biosynthesis in Arabidopsis.

Xu Yan Y Du Jinge J Hao Ruili R Ma Siqi S Ma Yujiao Y Zhou Gongke G Hu Ruibo R Li Shengjun S

Nature communications 20250430 1

Pectin is an abundant polysaccharide with essential roles in various biological processes. Despite considerable advances in understanding the regulatory mechanisms of pectin biosynthesis, the influence of phytohormones on this process remains unclear. Here we report that gibberellins (GA) promotes pectin biosynthesis in Arabidopsis. The DELLA proteins, as GA signaling repressors, interact with TRANSPARENT TESTA GLABRA2 (TTG2) and components of the MYB-bHLH-WD40 (MBW) complex, the key regulators ...[more]

PMID: 40307219

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Dataset Information

Candidate Arabidopsis MYB5-interacting proteins in developing siliques identified by IP-MS

Publications

Gibberellin signaling regulates pectin biosynthesis in Arabidopsis.

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