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Proteomic Analysis of Cotton Apoplastic Fluid (APF)

ABSTRACT: This project focuses on the proteomic analysis of apoplastic fluids (APFs) extracted from cotton (Gossypium hirsutum) leaves using an optimized infiltration-centrifugation protocol. The APFs, obtained through a meticulous process involving vacuum infiltration, drying, and two-step centrifugation, are free from cellular contamination and are important for in-depth protein profiling to understand leaf physiological functions and responses to environmental stimuli.

ORGANISM(S): Gossypium Hirsutum

SUBMITTER: Jinquan Huang

PROVIDER: PXD062792 | iProX | Tue Sep 02 00:00:00 BST 2025

REPOSITORIES: iProX

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Project description:This study introduced the use of cotton in 200µL tips, namely cotton HILIC as a convenient tool for enriching large-scale glycopeptides before MS analysis in this study. The optimal loading buffer for cotton HILIC was investigated by using mouse brain as a complex test sample. Subsequently, the performance of cotton HILIC was evaluated by comparing with other two commercial enrichment approaches, venusil HILIC and Oasis MAX in both mouse brain and seminal plasma. Moreover, capacity and recovery rate were evaluated using different milligram of cotton in 200µL tips. Thus, a simple, convenient, and cost-friendly cotton HILIC method was proposed to achieve highly selective intact glycopeptide profiling.

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