Proteomics

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Identification of GSDME-interacting proteins in HK-2 cells by immunoprecipitation-coupled LC-MS/MS analysis

ABSTRACT: This project aims to identify GSDME-associated proteins in human renal tubular epithelial (HK-2) cells using immunoprecipitation followed by LC-MS/MS. HK-2 cells overexpressing Flag-tagged GSDME were lysed, and protein complexes were enriched using anti-Flag antibody and Protein A/G magnetic beads. Protein lysates were analyzed by liquid chromatography-tandem mass spectrometry. Protein identification was performed using the UniProtKB human database. The dataset provides insights into the potential protein interactors of GSDME and their relevance to post-translational regulation.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jing Nie  

PROVIDER: PXD062902 | iProX | Sat Apr 12 00:00:00 BST 2025

REPOSITORIES: iProX

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Publications

Renal tubular GSDME protects cisplatin nephrotoxicity by impeding OGT-STAT3-S100A7A axis in male mice.

Chen Qingzhou Q   Sun Pengxiao P   Zhou Jiaxin J   Long Tantan T   Xiao An A   Liu Zhuoliang Z   Xu Shihui S   Lei Wenjing W   Zhang Rui R   Tian Jianwei J   Zhou Miaomiao M   Hu Zheng Z   Zhu Fengxin F   Nie Jing J  

Nature communications 20250724 1

Gasdermin E (GSDME) is known as a key executive protein of pro-inflammatory pyroptosis. However, the function diversity of GSDME needs further investigation. Here, we show that GSDME expression is downregulated in kidney tissues after cisplatin treatment without detectable N-terminal fragment. Global and tubule-specific Gsdme deficiency aggravates cisplatin-induced renal injury. Mechanistically, loss of GSDME in proximal tubular cells facilitates the recruitment of OGT to the CUL4B-DDB1-WDR26 E3  ...[more]

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