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Analysis of Nucleocytoplasmic Protein Separation in DPST-Crosslinked Human Neuroblastoma SY5Y Cells

ABSTRACT: Following DPST crosslinking of human neuroblastoma cells, nuclear-cytoplasmic fractionation was performed using hypotonic lysis to obtain cytoplasmic and nuclear protein compartments. Through systematic analysis of crosslinking mass spectrometry data, we elucidated how subcellular compartmentalized microenvironments regulate spatial distribution of protein conformations and topological architecture of interaction networks. This study reveals the heterogeneous principles governing dynamic protein assembly between nuclear and cytoplasmic compartments in neuroblastoma cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Lihua Zhang

PROVIDER: PXD062913 | iProX | Mon Apr 14 00:00:00 BST 2025

REPOSITORIES: iProX

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Dissecting In Vivo Cross-Linker Performance: Insights into Structural Dynamics and Molecular Rigidity.

Zhou Yuxuan Y Zhang Beirong B Zhong Bowen B Sun Min M Zhang Xu X Zhang Yukui Y Zhang Lihua L Zhao Qun Q Gong Zhou Z Liu Maili M

JACS Au 20250709 7

Understanding protein structure and dynamics is crucial to elucidating their biological functions. Recent advancements in experimental techniques and AI-driven predictions, such as AlphaFold, have significantly enhanced our ability to determine protein structures. However, most methods are based on in vitro studies, which often fail to capture the complexities of protein behavior in vivo. This study focuses on in vivo chemical cross-linking mass spectrometry (XL-MS) as a pro ...[more]

PMID: 40747017

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