A Novel Strategy to Rapidly Profile and Quantify High-Risk Host Cell Proteins using Integrated DDA-PRM-dMRM Mode

ABSTRACT: We developed a novel strategy using integrated DDA-PRM-dMRM to comprehensively profile and quantify high-risk HCPs in CHO cell-derived biotherapeutics. Firstly, we performed deep proteomic analysis of CHO cells using DDA-PASEF mode, constructing a high-quality spectral library covering all potential HCPs. Based on the constructed library, we validated the target list using PRM-PASEF and MRM modes.

ORGANISM(S): Cricetulus Griseus

SUBMITTER: Jinlan Zhang  

PROVIDER: PXD063690 | iProX | Sat May 03 00:00:00 BST 2025

REPOSITORIES: iProX