Proteomics

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Photocatalytic Proximity Labeling for Mapping Lysosomal Proteome


ABSTRACT: This project develops the photocatalytic lysosome-tracking proteome mapping (PLT-PM) method, which uses LysoTracker DND-26 as both a lysosomal tracker and visible-light photocatalyst. The method enables one-step, high-resolution, and low-disruption profiling of the lysosomal proteome without requiring genetic modification or custom dye synthesis. It was applied alongside LC-MS/MS to analyze lysosomal proteome changes during rapamycin and amino acid starvation-induced autophagy, offering a biocompatible and accessible platform to advance lysosome-related research.

ORGANISM(S): Homo Sapiens

SUBMITTER: Liang Zhang  

PROVIDER: PXD063691 | iProX | Sat May 03 00:00:00 BST 2025

REPOSITORIES: iProX

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