Project description:MTCH2 is a protein localized in the outer membrane of mitochondria. It belongs to the solute carrier 25 family, but its substrates or transporter function remains unknown. Previous research links MTCH2 to apoptosis induction, and Alzheimer’s disease, mitochondrial metabolism and dynamics, and MTCH2 has been shown to function as a protein insertase and scramblase. Moreover, MTCH2 is a significant regulator of adipocyte differentiation and lipid homeostasis. Genome-wide association studies have identified MTCH2 variants to be associated with increased body mass index, obesity, and diabetes risk. Thus, MTCH2 emerges as a promising candidate for modulating adipocyte function and whole-body energy metabolism, but its specific metabolic role in mature adipose tissues remains unexplored. In this study, we show that MTCH2 regulates mitochondrial function and whole-body energy expenditure by regulating lipid utilization in adipose tissue.
Project description:Background: The potential of Camellia oleifera meal peptides(PP) in the treatment of atherosclerosis and its potential mechanism need to be further clarified. Objective: the aim of this study was to clarify the effect of PP in the treatment of atherosclerosis and its potential mechanism. Methods: After cell modeling, it was used to detect the antioxidant, anti-inflammatory and lipid-lowering effects of PP in vitro. As mouse model was used to evaluate the anti atherosclerotic effect of PP in vivo. The changes of protein and metabolites after the treatment of atherosclerosis by PP were studied by liver proteomics, metabonomics and transcriptomics, and the possible mechanism of its regulation of lipid metabolism was clarified. Results:Using cell inflammation model, oxidative damage model and high-fat model, CCK8 test and qPCR detection showed that PP had antioxidant, anti-inflammatory and lipid-lowering effects. The animal results showed that the lipid deposition in the root of aortic valve and liver tissue was reduced, and the PP had the effect of alleviating atherosclerosis. Metabonomics and proteomics analysis showed that the activities of various enzymes related to lipid metabolism were changed after PP treatment, and the lipid metabolism pathway was changed. Transcriptomics analysis showed that AMPK pathway was changed. The experimental results showed that AMPK regulated lipid oxidation pathway to alleviate atherosclerosis in mice through acetyl CoA carboxylase (ACC) - CPT-1 pathway.
Project description:Despite a variety of seasoning ingredients in diets, little is known about their cooperative effect on animal metabolism. We fed rats a diet containing 30 wt.% instant noodle with a 26% fat-to-energy ratio for 30 days (N-group). Compared with rats that were fed the same diet without seasonings (C-group), the N-group showed lower liver triacylglycerol levels and higher fecal cholesterol levels. To assess the mechanisms underlying this phenotype, we conducted transcriptome analyses of the hypothalamic–pituitary axis (HP), liver and white adipose tissue (WAT). Our results suggest that these ingredients may affect lipid homeostasis via the HP axis.
Project description:Despite a variety of seasoning ingredients in diets, little is known about their cooperative effect on animal metabolism. We fed rats a diet containing 30 wt.% instant noodle with a 26% fat-to-energy ratio for 30 days (N-group). Compared with rats that were fed the same diet without seasonings (C-group), the N-group showed lower liver triacylglycerol levels and higher fecal cholesterol levels. To assess the mechanisms underlying this phenotype, we conducted transcriptome analyses of the hypothalamic–pituitary axis (HP), liver and white adipose tissue (WAT). Our results suggest that these ingredients may affect lipid homeostasis via the HP axis.
Project description:Despite a variety of seasoning ingredients in diets, little is known about their cooperative effect on animal metabolism. We fed rats a diet containing 30 wt.% instant noodle with a 26% fat-to-energy ratio for 30 days (N-group). Compared with rats that were fed the same diet without seasonings (C-group), the N-group showed lower liver triacylglycerol levels and higher fecal cholesterol levels. To assess the mechanisms underlying this phenotype, we conducted transcriptome analyses of the hypothalamic–pituitary axis (HP), liver and white adipose tissue (WAT). Our results suggest that these ingredients may affect lipid homeostasis via the HP axis.