The potential binding proteins of FOXQ1 were detected by mass spectrometry technology, laying the foundation for the subsequent in-depth study of its molecular mechanism

ABSTRACT: First, Flag-FOXQ1 was overexpressed in HCT-116 cells. Subsequently, the Flag-FOXQ1 complex was enriched via immunoprecipitation (IP) technology. The complex was then washed four times with phosphate-buffered saline (PBS), denatured in loading buffer by boiling, and separated via gel electrophoresis to obtain the protein components of the complex. Finally, mass spectrometry analysis was performed for identification.

ORGANISM(S): Homo Sapiens

SUBMITTER: Jin Bai  

PROVIDER: PXD064807 | iProX | Mon Jun 09 00:00:00 BST 2025

REPOSITORIES: iProX