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Screening of fiber-degrading bacteria and metabolic utilization rice straw

ABSTRACT: To increase the utilization rate of straw, the purpose of this study screened cellulose-degrading bacteria from the intestinal tract of termites by Congo red primary screening and filter paper degradation re-screening test, which was Cellulomonas iranensis (CE). The enzyme activities of endoglucanase, exoglucose and β glucosidase of them were measured at 0.097 U/mL, 0.316 U/mL, 0.110 U/mL. PS was also inoculated in the liquid medium with rice straw or glucose as the sole carbon source for14 days fermentation. Further transcriptomic and proteomic analyses revealed that rice straw cultured CE an upregulation of genes and a downregulation of genes, compared with glucose cultured PS.

ORGANISM(S): Cellulomonas Iranensis

SUBMITTER: Qing Zhang

PROVIDER: PXD065194 | iProX | Wed Jun 18 00:00:00 BST 2025

REPOSITORIES: iProX

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Screening and metabolic utilization of rice straw cellulose-degrading bacteria

Project description:In order to improve the utilization rate of straw, Cellomonas Iranensis (CE) , a cellulose-degrading bacterium, was isolated from the gut of termites by Congo red screening and filter paper degradation re-screening. The enzyme activities of endogenous glucosidase, exogenous glucosidase and β-glucosidase were determined at the levels of 0.097 u/mL, 0.316 u/mL and 0.110 u/mL. CE was inoculated into liquid medium with filter paper or glucose as the sole carbon source and fermented for 14 days. Further transcriptomic and proteomic analyses revealed that rice straw-cultured CE was characterized by gene upregulation and gene downregulation compared with glucose-cultured CE.

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dynamic SILAC NIH/3T3 treated with DMSO/CHX

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