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Identification of ATG7 as a protein ATG8ylation substrate

ABSTRACT: Identification of endogenous protein ATG8ylated ATG7 over 100kDa fractions by MS

ORGANISM(S): Homo Sapiens

SUBMITTER: Min Li

PROVIDER: PXD065606 | iProX | Sun Jun 29 00:00:00 BST 2025

REPOSITORIES: iProX

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Identification of ATG7 as a protein ATG8ylation substrate.

Xie Huazhong H Long Jiahui J Huang Yijun Y Wang Yao Y Liu Peiqing P Li Min M

Cell reports 20251103 11

Protein ATG8ylation is a post-translational modification where ubiquitin-like protein LC3/ATG8 forms covalent conjugation with cellular proteins, a process reversed by ATG4. In contrast to the well-characterized ATG8 lipidation/membrane ATG8ylation, research on protein ATG8ylation remains limited. In this study, we identify deconjugation-resistant LC3B Q116A and F80A/L82A mutants as tools for protein ATG8ylation. We demonstrate that protein ATG8ylation depends exclusively on ATG4, ATG3, and ATG7 ...[more]

PMID: 41187061

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