Proteomic sequencing on the renal cortices of wild-type and TRIM65 knockout diabetic kidney disease mice

ABSTRACT: The proteomic sequencing on the renal cortices of wild-type (WT) and TRIM65 knockout (KO) diabetic kidney disease (DKD) mice, focusing on the renal tubules, which are the predominant structures within the renal cortex. DKD mouse model: The streptozotocin (STZ)-induced diabetes model combined with unilateral nephrectomy was used to replicate the mice DKD model. Unilateral nephrectomy was under isoflurane anesthesia. Before established DKD mice model we established a baseline. In short, the mice were fed a high-fat diet for 2 weeks and then underwent unilateral nephrectomy. The high-fat diet was continued for another 2 weeks, and STZ (40 mg/kg/5 days) was intraperitoneally injected to the mice. Tail vein blood glucose was measured after 72 h. The establishment of the diabetes model was considered successful if fasting blood glucose ≥ 11.1 mmol/L or random blood glucose ≥ 16.7 mmol/L. Seven days after the STZ injection, blood glucose was measured again, and mice with stable blood glucose within 7 days were categorized as successfully established diabetic mice models. At 24 weeks after the induction of diabetes, mice were sacrificed, and their kidneys were harvested. Mice fed with normal chow without any manipulation were used as the control group.

ORGANISM(S): Mus Musculus

SUBMITTER: Zhen Liang  

PROVIDER: PXD065829 | iProX | Sun Jul 06 00:00:00 BST 2025

REPOSITORIES: iProX