Proteomics

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Protocol for structural analysis of amyloid fibrils using hydrogen/deuterium exchange mass spectrometry


ABSTRACT: Amyloid fibrils are common in many diseases. This protocol uses α-synuclein fibrils, a Parkinson’s disease hallmark, to demonstrate hydrogen-deuterium exchange mass spectrometry (HDX-MS) for structural analysis. It details fibril preparation, quality assessment, HDX labeling, digestion, and mass spectrometry. Steps for peptide mapping and data interpretation are included. This workflow serves as a practical guide for researchers, particularly new comers, to study insoluble protein aggregates with distinct conformations.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hu Zhou  

PROVIDER: PXD066123 | iProX | Mon Jul 14 00:00:00 BST 2025

REPOSITORIES: iProX

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