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Ex vivo lung perfusion enhances donor lung preservation in mice via Hippo signaling activation

ABSTRACT: Ex vivo lung perfusion (EVLP) is a promising technique that allows organ preservation and repair, increasing the number of marginal donor lungs for lung transplantation. This study aimed to establish an EVLP model in mice, and to unveil the molecular mechanisms responsible of EVLP beneficial effects. Methods: The mouse EVLP system was established, and mouse lungs were exposed to: (1) without ischemia or EVLP (control), (2) 45 min EVLP followed by 135 min cold ischemia (EVLP-CI), (3) 135 min cold ischemia followed by 45 min EVLP (CI-EVLP), and (4) 180 min cold ischemia (CI). At the end of the 3-hour lung preservation period, lungs were collected for proteomic profiling.

ORGANISM(S): Mus Musculus

SUBMITTER: Tian Jiang

PROVIDER: PXD066193 | iProX | Wed Jul 16 00:00:00 BST 2025

REPOSITORIES: iProX

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Ex vivo lung perfusion enhances donor lung preservation in mice via Hippo signaling activation.

Huang Renhui R Zhao Zhiwang Z Liu Sijia S Li Mei M Wang Ying Y Hu Yunfan Y Sun Tiantao T Duan Zhiyun Z Ren Changhao C Yang Xinyu X Zhang Shaoyuan S Jiang Tian T Yin Jun J Tan Lijie L

American journal of respiratory cell and molecular biology 20260301 3

<h4>Rationale</h4>Ex vivo lung perfusion (EVLP) is a promising technique that allows organ preservation and repair, while the molecular mechanisms remain unknown.<h4>Objectives</h4>This study aimed to establish a translational murine EVLP model and to unveil the molecular mechanisms responsible for EVLP beneficial effects.<h4>Methods</h4>We developed a murine EVLP system with four experimental groups: (1) without ischemia or EVLP (control), (2) 45 minutes of EVLP followed by 135 minutes cold isc ...[more]

PMID: 40996835

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