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Supporting data for manuscript: A modified fully automated dried blood spot sample preparation system and its application in steroid esters detection

ABSTRACT: This project focuses on developing an efficient and sensitive method for detecting steroid esters in dried blood spots (DBS) to support anti-doping efforts. By optimizing a fully automated DBS processing system with an independent desorption pump, we established a high-throughput screening method for 30 steroid esters and a confirmatory method for 26 targets using HPLC-HRMS. The method demonstrates low detection limits (as low as 0.05 ng/mL) and good reproducibility (RSD < 15%), and its performance was validated through blind samples, administration studies, and comparison with traditional IRMS. This approach provides a practical solution for rapid and accurate screening and confirmation of steroid esters, enhancing anti-doping capabilities.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xu Xin

PROVIDER: PXD067516 | iProX | Wed Aug 20 00:00:00 BST 2025

REPOSITORIES: iProX

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