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Comprehensive Human Red Blood Cell Proteome Analyses

ABSTRACT: Red blood cells (RBCs) are essential in carrying oxygen to human for maintenance of life and involved in many clinical disorders. Many proteomics studies have been done on RBCs, but the depths are insufficient, limiting better understanding of protein profiles of RBCs and molecular mechanisms of their diseases. With major advances that have been made in the mass spectrometry-based proteomics filed, here we have well prepared RBCs into the membrane and cytoplasmic fractions and performed in-depth proteomics analyses respectively. The two deep proteomics analyses involved extensive basic pH RPLC fractionation (~100 fractions collected) and individual analysis without concatenation, yielding identification of 4777 proteins in the RBC membrane fraction, 2350 proteins in the cytoplasmic fraction, and 5264 proteins in total, presenting almost the largest scale in the human RBC proteome analysis field. This comprehensive RBC proteome study will provide useful datasets for better understanding human RBCs and the molecular mechanisms of their involved diseases.

ORGANISM(S): Homo Sapiens

SUBMITTER: Bing Bai

PROVIDER: PXD067677 | iProX | Sun Aug 24 00:00:00 BST 2025

REPOSITORIES: iProX

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