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Identification and Characterization of Extracellular Vesicles Released from CSFV-Infected PK-15 Cells

ABSTRACT: Extracellular vesicles (EVs) are membrane-bound particles released by cells, capable of carrying diverse cytoplasmic constituents and influencing the physiological functions of both proximal and distant recipient cells. Because EVs can protect their cargo from immune surveillance, they are exploited by viruses to evade neutralizing antibodies. Classical swine fever virus (CSFV) is hypothesized to utilize EVs for dissemination among susceptible cells. Analyzing the composition of these virus-associated EVs can reveal critical steps in the viral life cycle. Therefore, we isolated EVs mediating CSFV transmission via differential centrifugation and size-exclusion chromatography, and characterized their molecular composition. This work establishes a foundation for investigating key mechanisms in the CSFV life cycle.

ORGANISM(S): Classical Swine Fever Virus

SUBMITTER: Zengjun Lu

PROVIDER: PXD067774 | iProX | Wed Aug 27 00:00:00 BST 2025

REPOSITORIES: iProX

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