Association Study on the Regulation of Muscle Development-Related Genes, Proteins, and Metabolites in Different Parts by Gastrointestinal Flora During Various Developmental Stages of Liaoning Cashmere Goats
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ABSTRACT:
ORGANISM(S): Capra Hircus
SUBMITTER: 
Zeying Wang
PROVIDER: PXD068711 | iProX | Mon Sep 22 00:00:00 BST 2025
REPOSITORIES: iProX
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Project description:<p> Introduction: Muscle growth and development in Liaoning cashmere goats (a dual-purpose breed for cashmere and meat) and its regulation by intestinal flora are key to improving meat production, yet relevant molecular mechanisms remain unclear.</p><p> Objectives: To reveal the molecular mechanism of muscle growth and development in Liaoning cashmere goats and the regulatory role of intestinal flora, thereby improving their meat production performance.</p><p> Methods: Muscle tissues (intercostal, hindlimb biceps femoris, longissimus dorsi) and digestive contents (rumen, small intestine, cecum, feces) from goats at 6, 12, 18 months were collected. Transcriptomics, proteomics, metabolomics, 16S rRNA sequencing, and multi-omics integration analysis were used.</p><p> Results: HBFM exhibited optimal development at 12 months of age, which was influenced by g_Lachnospiraceae_NK3A20_group in the small intestine. The HOXC10 gene drove myocyte proliferation, while the MYL3 gene and its encoded protein synergistically participated in muscle physiological changes, involving the cAMP signaling pathway. LDM development was more active at 12 months, regulated by g_Christensenellaceae_R-7_group in the rumen and cecum, leading to significant expression of the MEF2A gene and MYH6 protein. Additionally, the COL1A1/COL1A2 genes participated in intramuscular fat deposition through purine metabolism. ICM showed vigorous early proliferation at 6 months, regulated by g_Aeriscardovia in the small intestine, which promoted high expression of Actin. L-malic acid and L-serine affected muscle growth and water-holding capacity in this region. At 18 months, the GDI protein influenced fat deposition in ICM through the cofactor biosynthesis pathway. Multi-omics integration analysis revealed that microorganisms such as g_norank_f_UCG-011 were significantly associated with numerous metabolites, and genes like FKBP5 synergistically regulated muscle development with proteins such as Actin.</p><p><em>Conclusion:</em> The development of different muscle parts in Liaoning cashmere goats is coordinately regulated by specific genes, proteins, metabolites, and intestinal flora, providing key targets for targeted breeding to improve meat production performance.</p>
2025-10-30 | MTBLS12972 | MetaboLights
Project description:we used single-cell RNA sequencing (scRNA-seq) and computational models to identify 13 skin cell types in Liaoning Cashmere Goats. We also analyzed the molecular changes by Cell Trajectory Analysis in the development process and revealed the maturation process in gene expression profile in Liaoning Cashmere Goats. Weighted gene co-expression network analysis (WGCNA) explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results showed different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify, and prove the differential expression in the coarse type and the fine type of Liaoning Cashmere Goats. Therefore, CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat secondary hair follicle dermal papilla cells, our research will provide new insights into the mechanism of cashmere growth and cashmere fineness regulation by cells.
2021-08-25 | GSE182474 | GEO
Project description:Guard hair and cashmere undercoat are developed from primary and secondary hair follicle, respectively. Little is known about the gene expression differences between primary and secondary hair follicle cycling. In this study, we obtained RNA-seq data from cashmere and milk goats grown at four different seasons. We studied the differentially expressed genes (DEGs) during the yearly hair follicle cycling, and between cashmere and milk goats. WNT, NOTCH, MAPK, BMP, TGFβ and Hedgehog signaling pathways were involved in hair follicle cycling in both cashmere and milk goat. However, Milk goat DEGs between different months were significantly more than cashmere goat DEGs, with the largest difference being identified in December. Some expression dynamics were confirmed by quantitative PCR and western blot, and immunohistochemistry. This study offers new information sources related to hair follicle cycling in milk and cashmere goats, which could be applicable to improve the wool production and quality.
2019-07-19 | GSE84947 | GEO
Project description:cashmere goat gastrointestinal Metagenome Metagenome
| PRJNA1205530 | ENA
Project description:cashmere goat gastrointestinal microorganisms Raw sequence reads
| PRJNA1132486 | ENA
Project description:Small RNA-seq of exosomes derived from epithelial cells of skin tissue on the side of the body of Inner Mongolia cashmere goats at 55 days of fetal development
2025-11-07 | GSE281226 | GEO
Project description:Secondary hair follicles (SHFs) produce the cashmere of goats. In the present study, SHF clusters were isolated under the microscope by cutting out the epidermis and removing the connective tissues. RNA sequencing was performed on the collected SHFs. Through genome-wide transcriptomic analysis, we discovered and characterized differentially expressed molecular signatures across the annual growth cycle of cashmere and demonstrated their differential expression features and importance in cashmere growth.
2023-01-31 | GSE221472 | GEO
Project description:Cashmere fineness is an important index to evaluate cashmere quality. Liaoning Cashmere Goat (LCG) has a large cashmere production and long cashmere fiber. But the fineness is not enough,improving the fineness of cashmere is an important problem.Therefore, it is important to find genes involved in cashmere fineness that can be used in future in future endeavors aiming to improve this phenotype. With the continuous advancement of research, the regulation of cashmere fineness has made new development through high-throughput sequencing and genome-wide association analysis. It has been found that translatomics can identify genes associated with phenotypic traits. Through a translatomic analysis, the skin tissue of LCG sample groups differing in cashmere fineness was sequenced by Ribo-seq. With this data, we identified 529 differentially expressed genes between the sample groups among the 27197 expressed genes(expressed,not co-expressed). From these, 343 genes were up-regulated in the fine LCG group in relation to the coarse LCG group and 186 down-regulated in the same relationship.Through GO enrichment analysis and KEGG enrichment analysis of differential genes, the biological functions and pathways of differential genes can be found. In the GO enrichment analysis, 491 genes were significantly enriched, and the functional region was mainly in the extracellular region. In the KEGG enrichment analysis, the enrichment of human papillomavirus infection pathway was the most. We found that COL6A5 gene may affect cashmere fineness. The characteristics and expression analysis of COL6A5 gene may play an important role in the study of LCG cashmere fineness.
2021-11-05 | GSE186959 | GEO
Project description:Cashmere, also known as soft gold, is produced from secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stage fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also successfully characterized. Based on cell lineage inference, we revealed detailed transcriptional gene expression profiles during dermal and epidermal lineage cell fate decisions. These works together delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.
2020-10-18 | GSE144351 | GEO
Project description:This study investigates regulatory molecules and machanisms of cashmere growth by profiling dermal papilla cells (DPCs) from secondary (SHF) and primary hair follicles (PHF) during the early and mid-anagen phases in both male and female cashmere goats. A comprehensive genome-wide transcriptomic analysis was then performed to characterize the differentially expressed molecular signatures associated with follicle types (SHF vs. PHF), developmental phases, and sex.
2025-11-13 | GSE307628 | GEO