Association Study on the Regulation of Muscle Development-Related Genes, Proteins, and Metabolites in Different Parts by Gastrointestinal Flora During Various Developmental Stages of Liaoning Cashmere Goats
Ontology highlight
ABSTRACT:
ORGANISM(S): Capra Hircus
SUBMITTER: 
Zeying Wang
PROVIDER: PXD068711 | iProX | Mon Sep 22 00:00:00 BST 2025
REPOSITORIES: iProX
Similar Datasets
Project description:<p> Introduction: Muscle growth and development in Liaoning cashmere goats (a dual-purpose breed for cashmere and meat) and its regulation by intestinal flora are key to improving meat production, yet relevant molecular mechanisms remain unclear.</p><p> Objectives: To reveal the molecular mechanism of muscle growth and development in Liaoning cashmere goats and the regulatory role of intestinal flora, thereby improving their meat production performance.</p><p> Methods: Muscle tissues (intercostal, hindlimb biceps femoris, longissimus dorsi) and digestive contents (rumen, small intestine, cecum, feces) from goats at 6, 12, 18 months were collected. Transcriptomics, proteomics, metabolomics, 16S rRNA sequencing, and multi-omics integration analysis were used.</p><p> Results: HBFM exhibited optimal development at 12 months of age, which was influenced by g_Lachnospiraceae_NK3A20_group in the small intestine. The HOXC10 gene drove myocyte proliferation, while the MYL3 gene and its encoded protein synergistically participated in muscle physiological changes, involving the cAMP signaling pathway. LDM development was more active at 12 months, regulated by g_Christensenellaceae_R-7_group in the rumen and cecum, leading to significant expression of the MEF2A gene and MYH6 protein. Additionally, the COL1A1/COL1A2 genes participated in intramuscular fat deposition through purine metabolism. ICM showed vigorous early proliferation at 6 months, regulated by g_Aeriscardovia in the small intestine, which promoted high expression of Actin. L-malic acid and L-serine affected muscle growth and water-holding capacity in this region. At 18 months, the GDI protein influenced fat deposition in ICM through the cofactor biosynthesis pathway. Multi-omics integration analysis revealed that microorganisms such as g_norank_f_UCG-011 were significantly associated with numerous metabolites, and genes like FKBP5 synergistically regulated muscle development with proteins such as Actin.</p><p><em>Conclusion:</em> The development of different muscle parts in Liaoning cashmere goats is coordinately regulated by specific genes, proteins, metabolites, and intestinal flora, providing key targets for targeted breeding to improve meat production performance.</p>
2025-10-30 | MTBLS12972 | MetaboLights
Project description:Guard hair and cashmere undercoat are developed from primary and secondary hair follicle, respectively. Little is known about the gene expression differences between primary and secondary hair follicle cycling. In this study, we obtained RNA-seq data from cashmere and milk goats grown at four different seasons. We studied the differentially expressed genes (DEGs) during the yearly hair follicle cycling, and between cashmere and milk goats. WNT, NOTCH, MAPK, BMP, TGFβ and Hedgehog signaling pathways were involved in hair follicle cycling in both cashmere and milk goat. However, Milk goat DEGs between different months were significantly more than cashmere goat DEGs, with the largest difference being identified in December. Some expression dynamics were confirmed by quantitative PCR and western blot, and immunohistochemistry. This study offers new information sources related to hair follicle cycling in milk and cashmere goats, which could be applicable to improve the wool production and quality.
2019-07-19 | GSE84947 | GEO
Project description:we used single-cell RNA sequencing (scRNA-seq) and computational models to identify 13 skin cell types in Liaoning Cashmere Goats. We also analyzed the molecular changes by Cell Trajectory Analysis in the development process and revealed the maturation process in gene expression profile in Liaoning Cashmere Goats. Weighted gene co-expression network analysis (WGCNA) explored hub genes in cell clusters related to cashmere formation. Secondary hair follicle dermal papilla cells (SDPCs) play an important role in the growth and density of cashmere. ACTA2, a marker gene of SDPCs, was selected for immunofluorescence (IF) and western blot (WB) verification. Our results indicate that ACTA2 is mainly expressed in SDPCs, and WB results showed different expression levels. COL1A1 is a highly expressed gene in SDPCs, which was verified by IF and WB. We then selected CXCL8 of SDPCs to verify, and prove the differential expression in the coarse type and the fine type of Liaoning Cashmere Goats. Therefore, CXCL8 gene may regulate cashmere fineness. These genes may be involved in regulating the fineness of cashmere in goat secondary hair follicle dermal papilla cells, our research will provide new insights into the mechanism of cashmere growth and cashmere fineness regulation by cells.
2021-08-25 | GSE182474 | GEO
Project description:Small RNA-seq of exosomes derived from epithelial cells of skin tissue on the side of the body of Inner Mongolia cashmere goats at 55 days of fetal development
2025-11-07 | GSE281226 | GEO
Project description:Secondary hair follicles (SHFs) produce the cashmere of goats. In the present study, SHF clusters were isolated under the microscope by cutting out the epidermis and removing the connective tissues. RNA sequencing was performed on the collected SHFs. Through genome-wide transcriptomic analysis, we discovered and characterized differentially expressed molecular signatures across the annual growth cycle of cashmere and demonstrated their differential expression features and importance in cashmere growth.
2023-01-31 | GSE221472 | GEO
Project description:we evaluated the Arbas Cashmere goat, a species that is especially used in this type of research. The goats were exposed to an experimentally controlled short photoperiod to study the regulation of cyclical cashmere growth. Exposure to a short photoperiod extended the anagen phase of the Cashmere goat hair follicle to increase cashmere production. Based on assessments of tissue sections, it was observed that the short photoperiod significantly induced cashmere growth. A comparison of the differences in gene expression between the short photoperiod and the natural conditions using gene chip technology supported this conclusion. Using the gene chip data, we identified genes that showed altered expression under the short photoperiod compared to natural conditions, and it was found that these genes are involved in the biological processes of hair follicle growth, structural composition of the hair follicle, and the morphogenesis of the surrounding skin appendages. Based on their similar weights, health conditions and good reproductive performance in a group of the same strain, we chose 62-year-old female adult Arbas Cashmere goats as our research specimens and randomly divided them into a test group (T1, T2, T3) and a control group (C1, C2, C3).The goats in the test group were grazed, fed, and allowed to drink from 9:30-16:30 daily, and they were housed in a dark shed with good air conditions for the rest of the time. The temperature in the shed was 1 degree less than that outside, and the opacity was controlled at approximately 0.1 lux. The control group was grazed in natural conditions. We used regular management to care for all of the goats.
2015-11-11 | E-GEOD-74860 | biostudies-arrayexpress
Project description:Cashmere, also known as soft gold, is produced from secondary hair follicles in Cashmere goats and it’s therefore of significance to investigate the molecular profiles during Cashmere goat hair follicle development. However, our current understanding of the machinery underlying Cashmere goat hair follicle remains largely unexplored and researches regarding hair follicle development mainly used the mouse as a research model. To provides comprehensively understanding on the cellular heterogeneity and cell lineage cell fate decisions, we performed single-cell RNA sequencing on 19,705 single cells from induction (embryonic day 60), organogenesis (embryonic day 90) and cytodifferentiation (embryonic day 120) stage fetus Cashmere goat dorsal skin. Unsupervised clustering analysis identified 16 cell clusters and their corresponding cell types were also successfully characterized. Based on cell lineage inference, we revealed detailed transcriptional gene expression profiles during dermal and epidermal lineage cell fate decisions. These works together delineate unparalleled molecular profiles of different cell populations during Cashmere goat hair follicle morphogenesis and provide a valuable resource for identifying biomarkers during Cashmere goat hair follicle development.
2020-10-18 | GSE144351 | GEO
Project description:This study investigates regulatory molecules and machanisms of cashmere growth by profiling dermal papilla cells (DPCs) from secondary (SHF) and primary hair follicles (PHF) during the early and mid-anagen phases in both male and female cashmere goats. A comprehensive genome-wide transcriptomic analysis was then performed to characterize the differentially expressed molecular signatures associated with follicle types (SHF vs. PHF), developmental phases, and sex.
2025-11-13 | GSE307628 | GEO
Project description:This study investigates regulatory molecules and machanisms of cashmere growth by profiling dermal papilla cells (DPCs) from secondary (SHF) and primary hair follicles (PHF) during the early anagen and anagen phases in both male and female cashmere goats. A comprehensive genome-wide transcriptomic analysis was then performed to characterize the differentially expressed molecular signatures associated with follicle types (SHF vs. PHF), developmental phases, and sex.
2026-07-02 | GSE318952 | GEO
Project description:<p>This study integrated transcriptomics and metabolomics to analyse the molecular differences in the skin tissues of perennial long wool type (PT-LCG) and seasonal long wool type (ST-LCG) Liaoning cashmere goats to reveal the regulatory mechanisms underlying their differences in cashmere production. Histological observations showed no significant differences in the secondary hair follicle structure between the two types, but PT-LCG exhibited higher hair follicle activity during the catagen phase. Metabolomics analysis identified 92 differentially expressed metabolites (67 up-regulated and 25 down-regulated), primarily enriched in lipid metabolism, amino acid metabolism and energy metabolism pathways. The up-regulation of 3-hydroxypropionic acid and long-chain fatty acids may promote hair follicle growth through energy supply and membrane synthesis. Transcriptomic analysis revealed 145 differentially expressed genes (28 up-regulated and 117 down-regulated), significantly enriched in lipid metabolism, extracellular matrix (ECM), and inflammation-related pathways such as the PPAR and IL-17 signalling pathway. Multi-omics association analysis uncovered a synergistic regulatory network of ‘metabolite-receptor-gene’, indicating that lipid metabolism, energy supply and ECM remodelling are key factors in maintaining hair follicle activity in PT-LCG. This study provides potential targets for molecular breeding of cashmere goats and offers new insights into the regulatory mechanisms of mammalian hair follicle cycles.</p>
2025-11-27 | MTBLS13406 | MetaboLights