Dataset Information

Higher-energy collisional dissociation mass spectrometry fragmentation in cancer cells

ABSTRACT: mass spectrometric analysis of monosaccharide in cancer cells

ORGANISM(S): Homo Sapiens

SUBMITTER: Xuechen Li

PROVIDER: PXD069643 | iProX | Thu Oct 23 00:00:00 BST 2025

REPOSITORIES: iProX

ACCESS DATA

Publications

Higher-Energy Collisional Dissociation Mass Spectrometry Fragmentation Enables Distinguishing O-GlcNAc from Tn Antigen in Cancer Cells.

Chen Qiushi Q Cheng Wangzhirui W Li Can C Fung Yi Man Eva YME Wang Jiaqi J Liu Han H Li Xuechen X

Journal of the American Society for Mass Spectrometry 20260110 2

Protein glycosylation plays essential roles in various biological processes, and thus determining the glycan structure present on the protein is essential to comprehensively understand these events. However, distinguishing saccharide stereoisomers is challenging, especially when their structures are very similar and their molecular weight and potential glycosylation sites are identical. One representative example is O-linked β-<i>N</i>-acetylglucosamine (O-GlcNAc) and O-linked α-<i>N</i>-acetylg ...[more]

PMID: 41518202

Similar Datasets

Project description:Background. Despite major advances in cancer therapies, treatment failure remains a significant issue for a subset of patients due to therapy resistance and tumour recurrence. A key contributor to these failures is therapy-induced senescence, wherein damaged cells enter a stable cell cycle arrest while remaining metabolically active, contributing to chronic inflammation and cancer relapse. These senescent cells can promote chronic inflammation and disease relapse. However, the heterogeneous nature of senescence complicates its detection and therapeutic targeting. Methods. We employed a multimodal approach to profile metabolic alterations in senescent cancer cells. Senescent cells were induced either by doxorubicin or γ-irradiation, both widely employed strategies in anti-cancer treatment, across three human cancer cell lines: MCF7, HeLa, and TPC-1. Mitochondrial dysfunction was assessed through MitoTracker and JC-1 staining. Two-photon excitation fluorescence microscopy enabled label-free imaging of mitochondrial coenzymes NAD(P)H and FAD. Lipidomic changes were analyzed using MALDI mass spectrometry imaging following lipid extraction and matrix application. Additionally, transcriptomic profiling via RNA sequencing was performed on control, senescent, and engulfing-senescent MCF7 cells. Bioinformatic analyses included differential gene expression, gene set enrichment analysis, and pathway analysis using GO, KEGG, and Reactome databases Results. Across all models, senescent cells exhibited mitochondrial dysfunction, marked by altered NAD(P)H and FAD distribution and decreased mitochondrial membrane potential. Two-photon excited fluorescence imaging confirmed a broader intracellular redistribution of mitochondrial coenzymes. MALDI analyses revealed consistent lipid remodeling, particularly involving cardiolipin precursors such as PG(18:1/18:1) and PG(18:1/22:6). Transcriptomic profiling of senescent MCF7 cells revealed that a previously observed engulfing subpopulation is marked by enhanced oxidative metabolism and increased lipid catabolism, suggestive of an engulfing phenotype. Conclusion. Our findings delineate both conserved and divergent metabolic features across senescent cell states and highlight specific metabolic vulnerabilities. These insights offer potential avenues for the development of targeted senolytic strategies.

Dataset Information

Higher-energy collisional dissociation mass spectrometry fragmentation in cancer cells

Publications

Higher-Energy Collisional Dissociation Mass Spectrometry Fragmentation Enables Distinguishing O-GlcNAc from Tn Antigen in Cancer Cells.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets