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Identification of the PLK3-Mediated Phosphorylation Sites in NPM1

ABSTRACT: 293T cells were transfected with FLAG-tagged PLK1, PLK3 or a mock vector. At 48 h post-transfection, cell lysates were subjected to IP with an anti-NPM1 antibody. The resulting immunoprecipitates were analyzed by SDS-PAGE followed by sliver staining. Gel bands corresponding to NPM1 were excised, recovered, and analyzed by LC-MS to identify serine and threonine phosphorylation sites on NPM1.

ORGANISM(S): Homo Sapiens

SUBMITTER: Guoxin Liang

PROVIDER: PXD069861 | iProX | Thu Oct 23 00:00:00 BST 2025

REPOSITORIES: iProX

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