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SIRT5 ameliorates cardiac fibrosis via PCK2 de-succinylation–mediated metabolic reprogramming in cardiac fibroblasts

ABSTRACT: WT and Sirt5-/- CFBs were stimulated with TGF-β1 (10 ng/mL) for 36 hours. Cells were harvested, snap-frozen, and stored at -80℃. The experiment was independently repeated six times at different time points. Cells from each replicate were pooled to yield more than 3 mg of protein per group. Pooled WT and Sirt5-/- CFBs were submitted to PTM BIO (Zhejiang, China) for quantitative succinylomics analysis

ORGANISM(S): Mus Musculus

SUBMITTER: Yangxin Chen

PROVIDER: PXD070811 | iProX | Sat Nov 15 00:00:00 GMT 2025

REPOSITORIES: iProX

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