Photodynamic therapy with a novel photosensitizer inhibits BLM-induced pulmonary fibrosis in mice via MRC1-mediated pathway

ABSTRACT: For proteomic analysis, the middle lobe of right lung was dissected from each mouse. Tissues from individual mice were processed separately (not pooled) to account for biological variability, with n=3 per group used for this analysis. Lung tissue were homogenized in 600 μL of ice-cold lysis buffer (8 M urea supplemented with protease inhibitors). The homogenates were subjected to intermittent sonication (1 s on, 2 s off, for a total of 120 s) and subsequently centrifuged at 14,000 ×g for 20 min at 4°C to collect the supernatant. Protein concentration was determined using a bicinchoninic acid (BCA) assay. Protein quality and integrity were assessed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Further separation was performed using a RIGOL L-3000 rapid resolution high-performance liquid chromatography (RP-HPLC) system following the manufacturer's instructions.

ORGANISM(S): Rattus Rattus

SUBMITTER: Yumei Rong  

PROVIDER: PXD070823 | iProX | Sun Nov 16 00:00:00 GMT 2025

REPOSITORIES: iProX