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Dual Protease-Mediated Proteolysis Coupled with Ultrafiltration for System-Level Profiling of Drug Targets and Conformational Dynamics: cell level

ABSTRACT: Dual Protease-Mediated Proteolysis Coupled with Ultrafiltration for System-Level Profiling of Drug Targets and Conformational Dynamics

ORGANISM(S): Homo Sapiens

SUBMITTER: Jingchen Shi

PROVIDER: PXD071125 | iProX | Mon Nov 24 00:00:00 GMT 2025

REPOSITORIES: iProX

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Dual Protease-Mediated Proteolysis Coupled with Ultrafiltration for System-Level Profiling of Drug Targets and Conformational Dynamics.

Dong Xuefang X Shi Jingchen J Zhao Yanyan Y Zhao Liang L Li Xiuling X Liang Xinmiao X

Analytical chemistry 20260113 3

Characterizing drug-binding proteins and the subsequent changes in protein complexes/interactions is pivotal to fully understanding drug mechanisms. Here, we developed a novel modification-free approach, Dual Protease-Mediated Proteolysis Coupled with Ultrafiltration (DPMU), which integrates limited proteolysis, ultrafiltration fractionation, and quantitative mass spectrometry to systematically map drug-induced structural alterations. DPMU employs thermolysin (broad specificity; prefers hydropho ...[more]

PMID: 41529176

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