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Proteomic analysis of Enterococcus faecalis isolated from Golden Pomfret

ABSTRACT: The Enterococcus faecalis SZ07 was isolated from the intestine of Trachinotus ovatus，which can improve fish immunity.To screen the probiotic potential protein from culture supernatant of this isolate，Proteomic analysis using Liquid chromatography-mass spectrometry was performed to identify differential proteins in enriched hippocampal synaptic junction preparations. More than 1800 proteins were quantified

ORGANISM(S): Enterococcus Faecalis

SUBMITTER: Cai Jia

PROVIDER: PXD071442 | iProX | Tue Dec 02 00:00:00 GMT 2025

REPOSITORIES: iProX

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Project description:Background: While stressful events are recognized as an important cause of major depressive disorder (MDD), some individuals exposed to life stressors maintain normal psychological functioning. Although the molecular mechanism(s) underlying this phenomenon remain unclear, abnormal transmission and plasticity of hippocampal synapses may play a key role in the pathoetiology of MDD. Methods: A chronic mild stress (CMS) protocol was applied to separate susceptible and unsusceptible rat subpopulations. Proteomic analysis using an isobaric tag for relative and absolute quantitation (iTRAQ) coupled with tandem mass spectrometry was performed to identify differential proteins in enriched hippocampal synaptic junction preparations. Results: A total of 4318 proteins were quantified, and 89 membrane proteins were present in differential amounts. Of these, SynaptomeDB identified 81 (91%) having a synapse-specific localization. The unbiased profiles identified several candidate proteins within the synaptic junction that may be associated with stress vulnerability or insusceptibility. Subsequent functional categorization revealed that protein systems particularly involved in membrane trafficking at the synaptic active zone exhibited a positive strain as potential molecular adaptations in the unsusceptible rats. Moreover, through STRING and immumoblotting analysis, membrane-associated GTP-bound Rab3a and Munc18-1 appear to co-regulate syntaxin-1/SNAP25/VAMP2 assembly at the hippocampal presynaptic active zone of unsusceptible rats, facilitating SNARE-mediated membrane fusion and neurotransmitter release, and may be part of a stress-protection mechanism in actively maintaining an emotional homeostasis. Conclusions: The present results support the concept that there are a range of potential protein adaptations in the hippocampal synaptic active zone of unsusceptible rats, revealing new investigative targets that may contribute to a better understanding of stress insusceptibility.

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Proteomic analysis of Enterococcus faecalis isolated from Golden Pomfret

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