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First insight into the regulatory role of the glycoprotein hormones GPA2 and GPB5 in the small-spotted catshark (Scyliorhinus canicula L.) during spermiogenesis

ABSTRACT: This study is the first to report the effects of stimulation with recombinant ScGPA2, ScGPB5, and single-chain ScGPB5#ScGPA2 on vertebrate testicular tissue in vitro. A large-scale, untargeted proteomic analysis revealed for the first time that all three—ScGPA2, ScGPB5, and single-chain ScGPB5#ScGPA2—can modulate the abundance of 103 of the 7,619 identified proteins, including proteins related to spermiogenesis and immunity, as well as others that are less well characterized.

ORGANISM(S): Mus Musculus

SUBMITTER: Sourdaine Pascal

PROVIDER: PXD071493 | iProX | Wed Dec 03 00:00:00 GMT 2025

REPOSITORIES: iProX

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First insight into the regulatory role of the glycoprotein hormones GPA2 and GPB5 in the small-spotted catshark (Scyliorhinus canicula L.) during spermiogenesis.

Jeanne Fabian F Pilet Stanislas S Bernay Benoît B Sourdaine Pascal P

Journal of proteomics 20260220

Glycoprotein hormones alpha 2 (GPA2) and beta 5 (GPB5) are considered to be ancestral members of the glycoprotein hormone (GPH) family. Despite their potential roles in regulating thyroxine production, the immune response, and ovarian function, their roles in the testis remains largely unknown. To further explore this regulation, catshark testicular explants containing late spermatid stages were treated in vitro with recombinant GPA2, GPB5 and single-chain GPB5#GPA2. The comparative proteomic an ...[more]

PMID: 41724374

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