Mass Spectrometry-based Profiling of GALNT1-associated Protein Complexes Isolated via Co-Immunoprecipitation

ABSTRACT: This project identifies GALNT1-interacting proteins in primary fibroblast-like synoviocytes (RASFs) derived from rheumatoid arthritis patients using co-immunoprecipitation (co-IP) coupled with mass spectrometry (MS). Briefly, RASF lysates were incubated with anti-GALNT1 antibody to capture GALNT1-associated protein complexes, and the immunoprecipitated proteins were analyzed by MS. Raw data were processed with Proteome Discoverer against the UniProt human proteome database for protein identification/quantification, with high-confidence hits filtered by PSM scores, sequence coverage, and fold enrichment vs. IgG controls.

ORGANISM(S): Homo Sapiens

SUBMITTER: Yaoyao Zou  

PROVIDER: PXD071709 | iProX | Mon Dec 08 00:00:00 GMT 2025

REPOSITORIES: iProX