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SP3-PELSA: A Rapid and Sensitive Approach for Studying Protein-Ligand Interaction with Limited Samples

ABSTRACT: SP3-PELSA, a modified peptide-centric local stability assay integrated with SP3 sample preparation, enables fivefold faster processing, enhanced peptide recovery (especially for hydrophobic peptides and low-amount samples), and superior performance in drug target discovery, holding great potential for large-scale applications and studies on membrane proteins using scarce clinical samples.

ORGANISM(S): Homo Sapiens

SUBMITTER: Xumin Zhang

PROVIDER: PXD071732 | iProX | Wed Dec 10 00:00:00 GMT 2025

REPOSITORIES: iProX

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SP3-PELSA: A rapid and sensitive approach for studying protein-ligand interaction with small-amount samples.

Chen Jiaqin J Hu Guangxu G Song Jie J Huang Lin L Zhang Xumin X

Analytica chimica acta 20260324

Deciphering protein-ligand binding events is essential for unraveling biological pathways and advancing drug development. The recently developed peptide-centric local stability assay (PELSA) has demonstrated superior performance compared to other mainstream approaches. However, the conventional PELSA approach relies on filter-based peptide recovery, which results in prolonged processing time and relatively low yields, particularly when operating with limited starting material. To address these l ...[more]

PMID: 41997672

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