Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic profiling of boar testis, spermatozoa and seminal plasma under chronic heat stress following LRY15 supplementation

ABSTRACT: This project presents a mass spectrometry–based proteomic dataset investigating the effects of LRY15 supplementation on boar reproductive tissues and fluids under heat stress conditions. Testis, spermatozoa, and seminal plasma samples were collected from boars exposed to heat stress with or without LRY15 intervention. Proteins were extracted from each sample type and analyzed by LC–MS/MS to characterize global proteomic profiles. The resulting dataset provides a comprehensive resource for exploring molecular alterations in male reproductive tissues and seminal components associated with heat stress and probiotic supplementation in pigs.

ORGANISM(S): Sus Scrofa Domesticus

SUBMITTER: Yong Zhao

PROVIDER: PXD072026 | iProX | Tue Dec 16 00:00:00 GMT 2025

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Expression data from Human seminal vesicles

Project description:The human seminal plasma is a potential source of biomarkers for male reproductive disorders. A tissue-profiling analysis of the main organs participating in the secretion of this body fluid was conducted to identify tissue-specific genes along the male reproductive tract. Total RNA from non pathological Human seminal vesicles were extracted and hybridized on Affymetrix microarrays. Expression signals in seminal vesicles (present dataset), prostates (GEO; GSE7307), epidydimises (GEO; GSE7808) and testicular samples (Arrayexpress; E-TABM-130) were compared to identify genes that are detected in one of these organs only.

2010-06-08 | E-GEOD-17340 | biostudies-arrayexpress

A single cell atlas of the mouse seminal vesicle

Project description:During mammalian reproduction, sperm are delivered to the female reproductive tract bathed in a complex medium known as seminal fluid, which plays key roles in signaling to the female reproductive tract and in nourishing sperm for their onwards journey. The majority of seminal fluid is produced by a somewhat understudied organ known as the seminal vesicle. Here, we report a single-cell RNA-Seq atlas of the mouse seminal vesicle, generated using tissues obtained from 23 mice of varying ages, exposed to a range of dietary challenges. We define the transcriptome of the secretory epithelial cells in this tissue, identifying a relative homogeneous population of the epithelial cells responsible for producing the majority of seminal fluid. We also define the immune cell populations – including large populations of macrophages, dendritic cells, T cells, and NKT cells – which have the potential to play roles in producing various immune mediators present in seminal plasma. Together, our data provide a resource for understanding the composition of an understudied reproductive tissue with potential implications for paternal control of offspring development and metabolism.

2024-05-16 | GSE267191 | GEO

Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide

Project description:The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection.

2021-09-14 | GSE183632 | GEO

Expression data from Human seminal vesicles

2010-06-01 | GSE17340 | GEO

Protein identification of spermatozoa and seminal plasma in bottlenose dolphin (Tursiops truncatus)

Project description:Proteins play an important role in many reproductive functions such as sperm maturation and physiology, sperm transit in the female genital tract or sperm-oocyte interaction. However, little information concerning reproductive features is available in the case of aquatic animals. The present study aims to characterize the proteome of both spermatozoa and seminal plasma of bottlenose dolphins (Tursiops truncatus) as a model organism for cetaceans. Ejaculate samples were obtained voluntarily from two trained dolphins housed in an aquarium. Spermatozoa and seminal plasma were analyzed by means of proteomic analyses using a LC-MS/MS and a list with the gene symbols corresponding to each protein was submitted to DAVID database. A total of 423 and 307 proteins were identified in spermatozoa and seminal plasma, respectively, and the samples shared 111 proteins. Furthermore, 70 proteins were identified as involved in reproductive processes, 39 in spermatozoa, and 31 in seminal plasma. The top-5 most abundant proteins were also identified in these samples: AKAP3, ODF2, TUBB, GST3, ROPN1 for spermatozoa and CST11, LTF, ALB, HSP90B1, PIGR for seminal plasma. In conclusion, this proteomic study provides the first characterization of proteomic expression patterns in cetacean sperm and seminal plasma, opening a window to future research on the discovery of biomarkers, analysis of conservation capacity or additional applications in the field of assisted reproduction technologies.

2021-06-14 | PXD024588 | Pride

Downregulation of eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with equine arteritis virus long-term persistent infection

Project description:We have characterized miRNAs associated with equine seminal exosomes, and identified seminal exosomes eca-mir-128 to be specifically downregulated during equine arteritis virus long-term persistent infection in the reproductive tract of the stallion

2018-02-12 | GSE108180 | GEO

Unlocking Oocyte Potential: L-Asp's Protective Role Against Heat-Induced Meiotic Disruption

Project description:Global warming has made heat stress (HS) an unavoidable challenge in livestock production, significantly impacting animal feed intake, growth, reproduction, and health. Germ cells, including oocytes, are particularly sensitive to HS, with impaired oocyte maturation being a primary cause of reproductive dysfunction. This study examines the effects of HS on porcine oocyte meiotic maturation and evaluates the protective role of L-aspartic acid (L-Asp) supplementation during this process. Here, we report that L-Asp supplementation improves the quality of porcine oocytes under heat stress. More specifically, we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles, including the spindle assembly, chromosome structure and mitochondrial function. Moreover, heat stress triggered the overproduction of reactive oxygen species (ROS) and caused DNA damage, resulting in apoptosis in oocytes and halting embryonic development. Importantly, our findings showed that L-Asp supplementation during heat stress alleviated the meiotic defects in porcine oocyte maturation. These findings demonstrate that L-Asp supplementation is a beneficial strategy for improving oocyte quality under HS by facilitating both nuclear and cytoplasmic maturation. This approach holds promise as a practical strategy for mitigating the reproductive challenges posed by global warming in livestock production.

2025-05-31 | GSE297045 | GEO

Maternal Probiotic Supplementation Effects on the Placenta-Brain Axis

Project description:The rate of probiotic usage by pregnant women in the US and Canada ranges from 1.3 to 3.6 %. Probiotic supplements are available without a prescription and have gained currency in treating a variety of ailment ranging from reducing risk of constipation, diarrhea, other gastrointestinal conditions, eczema, pre-term birth, and prevent adverse pregnancy outcomes, including gestational diabetes mellitus (GDM) and depression/anxiety. Three possible mechanisms by which maternal probiotic supplementation might influence the placenta are through 1) directly impacting possible bacteria residing in the placenta (placenta microbiome), 2) altering bacterial metabolites produced by gut microbiota within the mother that induce placental changes, and 3) maternal probiotics might affect the composition of the bacteria within the maternal gut that affects her immune cells and their responses to the heterologous placenta. For the second potential mechanism, bacterial metabolites that might influence placenta include short chain fatty acids (SCFAs), polyamines (PAs), and Vitamins B9 (Folic Acid) and 12 (Cobalamin), among others. This project aims to determine the effects maternal probiotic supplementation in mice might have on the fetal placenta. With the number of women taking over probiotic supplements increasing, further research is needed to determine how these bioactive agents may affect the placenta and health of the offspring.

2025-09-26 | GSE309308 | GEO

Seminal plasma vaginal recovery

Project description:Seminal plasma

| PRJEB108823 | ENA

Expression data from porcine endometrial epithelial cells induced by seminal exosomes

Project description:Purpose: Seminal fluid (SF) is considered as a regulator on environment establishing in female reproductive tract of various mammal species without mechanism elucidated completely. This study aimed to identified the function of exosome isolated from SF on porcine endometrium. Methods: Total RNA profiles of porcine endometrial epithelial cells with seminal exosomes treatment (EXO.) or not (CTRL.) were generated by deep sequencing, in triplicate, using HiSeq 2500. Trimmed sequences were analyzed on the basis of the TopHat/Cufflinks pipeline based on the porcine genome and reference annotations obtained from Ensembl genome browser (https://www.ensembl.org). Results: 10916 transcripts, from which 70 genes were up-regulated and 114 genes were down-regulated with q-values less than 0.05 in EECs with seminal exosome treatment compared to those without exosome treatment. Conclusions: Through the gene ontology (GO) biological process and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis which revealed significantly enriched or decreased molecular functions, Our work demonstrated that exosomes derived from porcine seminal fluid induced endometrial immune and inflammatory response via CCL20 and AMCFII regulation specifically, which may facilitate sperm cells survival and activation, and resulting in appropriate environment required for fertilization and even implantation.

2017-10-26 | GSE106173 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data