Dataset Information

Comprehensive proteomics and phosphorylation analysis revealed the negative role of VaVIP1 in cold tolerance of grapevine.

ABSTRACT: Grapevine, a globally important fruit crop. Low temperature limits grapevine yield, quality and geographical distribution. Protein post-translational modification is involved in cold tolerance of grapevine. In order to cultivate new varieties of grapevine cold tolerance, we conducted an integrated proteomic and phosphoproteomic analysis of Vitis amurensis, identifying widespread changes involving 4,797 proteins, 10,104 phosphorylation sites and 56 motif sequences. Cold treatment resulted in 165 differential phosphorylation proteins and 635 differential phosphorylation sites. GO enrichment showed that differential phosphorylation proteins were mainly concentrated in biological processes, cellular components and molecular functions. Functional enrichment highlighted critical roles for metabolic and signaling pathways, including phenylpropanoid biosynthesis and MAPK cascades. From this dataset, we focused on the bZIP transcription factor VaVIP1 and uncovered its negative regulatory role in cold tolerance. VaVIP1 overexpression can make the grapevine damage more severe, as evidenced by increased oxidative stress, membrane damage, and attenuated osmotic adjustment. On the molecular mechanism, VaVIP1 overexpression suppressed the cold-induced activation of key genes in the CBF-regulatory pathway. Our work not only expands the phosphoproteomic resource for plants but also delineates a novel mechanism where VaVIP1 acts as a negative regulator to cold tolerance, providing a valuable theoretical foundation for improving cold tolerance in grapevine.

ORGANISM(S): Vitis Vinifera

SUBMITTER: Xiuming Zhang

PROVIDER: PXD072186 | iProX | Thu Dec 18 00:00:00 GMT 2025

REPOSITORIES: iProX

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A comprehensive proteomics and phosphorylation analysis reveals the negative role of VaVIP1 in cold tolerance in grapevine.

Qi Xingcheng X Zhang Xinjie X Wang Junjie J Shi Xuerong X Yang Shijin S Hao Xinyi X Xu Weirong W Yao Wenkong W Zhang Xiuming X

Plant science : an international journal of experimental plant biology 20260626

Grapevine is an economically important fruit crop worldwide and is susceptible to low temperature stress. Protein post-translational modifications play a crucial role in grapevine cold tolerance. Here, we performed integrated proteomic and phosphoproteomic analyses of Vitis amurensis, identifying widespread phosphorylation alterations across 4797 proteins and 10,104 phosphorylation sites distributed in 56 motifs. Cold treatment induced 165 differential phosphorylation proteins and 635 differenti ...[more]

PMID: 42362162

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Dataset Information

Comprehensive proteomics and phosphorylation analysis revealed the negative role of VaVIP1 in cold tolerance of grapevine.

Publications

A comprehensive proteomics and phosphorylation analysis reveals the negative role of VaVIP1 in cold tolerance in grapevine.

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