Multi-omics profiling of hepatopancreas of ridgetail white

ABSTRACT: With the intensification of global climate change and human activities, and in interac-tion with regional topography, salinization of soils and waters has intensified, posing a major ecological and environmental challenge worldwide. Here, we integrated histol-ogy, transmission electron microscopy, RNA sequencing (RNA-seq) and da-ta-independent acquisition (DIA)- based proteomics to profile hepatopancreas re-sponses of Exopalaemon carinicauda during acute sulfate stress (48 h). Sulfate exposure disrupted tubular architecture and organelle integrity, consistent with early cellular injury. Multi-omics analyses revealed metabolic reprogramming marked by sup-pressed glycolysis (e.g., HK2, ENO) and enhanced oxidative phosphorylation (e.g., ATP5F1B), together with activation of calcium signaling (e.g., SLC8A1, ADCY9) and reinforcement of antioxidant/one-carbon and glucose-branch pathways (e.g., SHMT2, PGAM2). These coordinated transcript–protein changes indicate a shift from rapid cytosolic ATP supply to mitochondrial ATP production while buffering Ca²⁺ overload and reactive oxygen species. Collectively, our results delineate the physiological and molecular adjustments that enable E. carinicauda to cope with sulfate conditions and provide mechanistic targets for selective breeding and water-quality management in saline–alkaline aquaculture.

ORGANISM(S): Palaemon Carinicauda

SUBMITTER: Wenjun Shi  

PROVIDER: PXD072331 | iProX | Tue Dec 23 00:00:00 GMT 2025

REPOSITORIES: iProX