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Doubling Proteome Coverage in Spatial Proteomics: MOFs-assisted Method for Trace Tissue Analysis

ABSTRACT: Spatial proteomics of trace tissues isolated via LCM is limited by insufficient protein identification depth and coverage. We introduce a qMOFs-assisted protein preparation workflow into LCM-spatial proteomics, doubling the number of identified proteins (100 μm × 100 μm mouse myocardium) with high reproducibility. Building on this enhancement, we uncover cardiac aging-related cytoskeletal destabilization and mitochondrial dysregulation in perivascular myocardium, identifying Rack1 as a key node.

ORGANISM(S): Mus Musculus

SUBMITTER: Xianting Ding

PROVIDER: PXD072548 | iProX | Tue Dec 30 00:00:00 GMT 2025

REPOSITORIES: iProX

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