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Genome-wide identification and proteomic analysis of the wheat ABCG family reveals a pivotal role of TaABCG7B-8 in fertility conversion of a photoperiod-temperature sensitive genic male sterility wheat

ABSTRACT: Photoperiod- and temperature-sensitive genic male sterility (PTGMS) plays a central role in two-line hybrid wheat breeding, yet its underlying molecular mechanisms remain incompletely understood. In this study, we performed a genome-wide identification of the ATP-binding cassette subfamily G (ABCG) genes in wheat and systematically investigated their potential roles in fertility conversion. Comparative proteomic analyses were conducted using anthers from the PTGMS wheat line under sterile and fertile conditions to characterize protein abundance changes associated with fertility transition. The proteomics data revealed that TaABCG7B-8 is significantly associated with fertility conversion, suggesting its pivotal role in anther development and pollen formation. These mass spectrometry-based proteomics datasets provide valuable resources for elucidating the regulatory network underlying PTGMS in wheat and for further functional characterization of ABCG family members in male reproductive development.

ORGANISM(S): Triticum Aestivum

SUBMITTER: Liping Zhang

PROVIDER: PXD072615 | iProX | Sun Jan 04 00:00:00 GMT 2026

REPOSITORIES: iProX

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Project description:Background: Pepper (Capsicum annuum L.) is a major cash crop throughout the world. Male sterility is an important characteristic in crop species that leads to a failure to produce functional pollen, and it has crucial roles in agricultural breeding and the utilization of heterosis. Objectives: In this study, we identified many crucial factors and important components in metabolic pathways in anther and pollen development, and elucidated the molecular mechanism related to pollen abortion in pepper. Methods: Pepper pollen was observed at different stages to detect the characteristics associated with male sterility and fertility. The phytohormone and oxidoreductase activities were detected in spectrophotometric and redox reaction assays, respectively. Proteins were extracted from male sterile and fertile pepper lines, and identified by TMT/iTRAQ (Tandem mass tags/isobaric tags for relative and absolute quantitation) and LC-MS/MS (liquid chromatograph-mass spectrometer) analysis. Differentially abundant proteins (DAPs) were analyzed based on Gene Ontology annotations and the Kyoto Encyclopedia of Genes and Genomes database according to |fold change)| > 1.3 and P value < 0.05. DAPs were quantified in the meiosis, tetrad, and binucleate stages by parallel reaction monitoring (PRM). Results: In this study, we screened and identified one male sterile pepper line with abnormal cytological characteristics in terms of pollen development. The peroxidase and catalase enzyme activities were significantly reduced and increased, respectively, in the male sterile line compared with the male fertile line. Phytohormone analysis demonstrated that the gibberellin, jasmonic acid, and auxin contents changed by different extents in the male sterile pepper line. Proteome analysis screened 1645 DAPs in six clusters, which were mainly associated with the chloroplast and cytoplasm based on their similar expression levels. According to proteome analysis, 45 DAPs were quantitatively identified in the meiosis, tetrad, and binucleate stages by PRM, which were related to monoterpenoid biosynthesis, and starch and sucrose metabolism pathways. Conclusions: We screened 1645 DAPs by proteomic analysis and 45 DAPs were related to anther and pollen development in a male sterile pepper line. In addition, the activities of peroxidase and catalase as well as the abundances of phytohormones such as gibberellin, jasmonic acid, and auxin were related to male sterility. The results obtained in this study provide insights into the molecular mechanism responsible for male sterility and fertility in pepper.

Dataset Information

Genome-wide identification and proteomic analysis of the wheat ABCG family reveals a pivotal role of TaABCG7B-8 in fertility conversion of a photoperiod-temperature sensitive genic male sterility wheat

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