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Identification of p72-interacting proteins in porcine alveolar macrophages

ABSTRACT: To explore the potential mechanisms regulating p72 stability, we performed co-immunoprecipitation using an anti-p72 antibody in ASFV-infected PAMs, followed by mass spectrometry analysis to identify host factors interacting with p72.

ORGANISM(S): Sus Scrofa Domesticus

SUBMITTER: Dongming Zhao

PROVIDER: PXD073232 | iProX | Sun Jan 18 00:00:00 GMT 2026

REPOSITORIES: iProX

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Cyclophilin A stabilizes the capsid protein p72 to facilitate African swine fever virus replication.

Kong Hui H Yang Liqin L Zhang Zhenjiang Z Zhang Ying Y Liu Linlin L Wang Wan W Song Linfei L Zhu Yuanmao Y Sun Encheng E Li Fang F Bu Zhigao Z Sheng Xiangpeng X Zhao Dongming D

Nature communications 20260307 1

African swine fever virus (ASFV) is a large DNA virus that poses a major threat to the global swine industry. Its virion is encapsulated by an icosahedral capsid predominantly composed of the structural protein p72, which constitutes approximately one-third of the total virion mass. Despite its abundance, the mechanisms regulating p72 stability remain poorly understood. Here, we demonstrate that host-mediated stabilization of p72 is essential for efficient ASFV replication. Mass spectrometry of ...[more]

PMID: 41794866

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