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Simultaneous profiling of phosphorylation and S-nitrosylation during maize seed germination

ABSTRACT: We developed a phosphate-affinity tag-switch (PAT-switch) proteomics workflow enabling simultaneous, site-specific profiling of phosphorylation and S-nitrosylation during maize seed germination.

ORGANISM(S): Zea Mays

SUBMITTER: Shijuan Yan

PROVIDER: PXD073327 | iProX | Wed Jan 21 00:00:00 GMT 2026

REPOSITORIES: iProX

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Temporal dynamics of protein phosphorylation and S-nitrosylation direct maize seed germination programs.

Zhang Wenyang W Wang Yanjiao Y Li Wenyan W Wei Yan Y Wu Shaowen S Zhuo Danting D Ye Mingyang M Chen Yuanyuan Y Huang Wenjie W Li Yang Y Xu Kainan K Lai Jianbin J Fernie Alisdair R AR Yan Shijuan S

The Plant journal : for cell and molecular biology 20260401 1

Seed germination directly influences seedling establishment and ultimately crop yield. While hormonal and transcriptional regulations of germination are well characterized, the role of post-translational modifications (PTMs) remains largely unexplored. In this resource article, we developed a phosphate-affinity tag-switch (PAT-switch) proteomics workflow enabling simultaneous, site-specific profiling of phosphorylation and S-nitrosylation during maize seed germination. Using this strategy, we id ...[more]

PMID: 41911598

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