Identification of the Mitochondrial USP30-Flag Interacting Proteome
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ABSTRACT: Based on our research on the regulation of mitochondrial quality control and the functional mechanisms of the deubiquitinase USP30; here, we utilized HeLa cells stably expressing USP30-Flag to systematically identify its interacting proteome through affinity purification-mass spectrometry (AP-MS). This screening successfully enriched a series of high-confidence proteins that potentially interact with USP30 within the mitochondrial environment. The experimental verification of candidate proteins through co-immunoprecipitation highlighted the robust capability of this AP-MS strategy in capturing functional interactors, providing a molecular basis for further understanding USP30-mediated regulation of mitochondrial proteostasis.
ORGANISM(S): Homo Sapiens
SUBMITTER:
Zigang Li
PROVIDER: PXD073831 | iProX | Fri Jan 30 00:00:00 GMT 2026
REPOSITORIES: iProX
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